College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Int J Biol Macromol. 2019 Aug 1;134:413-421. doi: 10.1016/j.ijbiomac.2019.05.049. Epub 2019 May 9.
A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper. Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs. The prepared composite hydrogel films were characterized using FTIR, SEM, rheology measurement, mechanical and swelling tests. Our results showed that the well dispersed spherical CS microspheres endowed hydrogel film increased mechanical properties and swellability. In vitro drug release displayed an accelerated release under weak acidic or reducing media. The in vitro cytotoxicity and cell apoptosis studies showed the drug-loaded composite film caused enhanced inhibition to HepG2 cells in presence of glutathione (GSH). Moreover, the film has exhibited excellent antimicrobial efficacy against E.coli and Staphylococcus aureus. Therefore, our simple and effective dual drug-loaded system has the potential to be used for cancer therapy in the future.
本文采用二硫交联剂半胱胺盐酸盐（CYS）将壳聚糖（CS）微球掺入羧甲基纤维素（CMC）水凝胶中，制备了一种氧化还原响应型复合水凝胶膜的简单方法。两种药物，一种是抗癌药物 5-氟尿嘧啶（5-FU）和一种是抗生素盐酸四环素（TH）被用作模型药物。采用傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、流变学测量、机械和溶胀试验对制备的复合水凝胶膜进行了表征。我们的结果表明，分散良好的球形 CS 微球赋予水凝胶膜增加了机械性能和溶胀性。体外药物释放显示在弱酸性或还原介质下具有加速释放。体外细胞毒性和细胞凋亡研究表明，在谷胱甘肽（GSH）存在的情况下，载药复合膜对 HepG2 细胞的抑制作用增强。此外，该膜对大肠杆菌和金黄色葡萄球菌表现出优异的抗菌功效。因此，我们简单有效的双载药系统具有未来用于癌症治疗的潜力。
