School of Food Science & Engineering, South China University of Technology, Guangzhou 510641, PR China; College of Life and Geographic Sciences, Kashi University, Kashi 844000, PR China; Key Laboratory of Biological Resources and Ecology of Pamirs Plateau of Xinjiang Uygur Autonomous Region, Kashi University, Kashi 844000, PR China.
School of Food Science & Engineering, South China University of Technology, Guangzhou 510641, PR China.
Int J Biol Macromol. 2020 Nov 15;163:1518-1528. doi: 10.1016/j.ijbiomac.2020.07.272. Epub 2020 Aug 6.
A natural hydrogel film was prepared using carboxymethyl cellulose (CMC), cellulose nanocrystals (CNC), and hydroxypropyl β cyclodextrin (HP-β-CD) as reactants and citric acid as the cross-linking agent and used for the controlled release of neohesperidin-copper(II)(NH-Cu (II)). The hydrogel film was characterized by ATR-FTIR, XRD, TGA and DSC. The film showed controlled swelling behavior; the release behavior of NH-Cu(II) from the hydrogel film was also investigated in different solutions including distilled water, various salt solutions including 0.9% NaCl, and solutions having different pH values. Thiazolyl blue tetrazolium bromide assay and relative growth rates were adopted to evaluate the biocompatibility and cytotoxicity of the prepared hydrogel films. The results indicated that the expansion kinetics followed Fickian diffusion and Schott's second-order kinetics model. The hydrogel film exhibited enhanced mechanical properties and improved thermal stability at high temperatures due to the addition of CNC, with the amount of added CNC affecting the swelling ratio, salt sensitivity, and pH sensitivity of the hydrogel film in different solutions. Additionally, the CNC largely improved the loading and encapsulation efficiency of the hydrogel films, with the optimal CNC addition amount being 4% which yielded a loading amount of 753.75 mg/g and an accumulated release rate of 85.08%. The hydrogel film with proven cell compatibility and non-cytotoxicity can potentially be used as a drug delivery and controlled release material.
采用羧甲基纤维素（CMC）、纤维素纳米晶体（CNC）和羟丙基-β-环糊精（HP-β-CD）为反应物，柠檬酸为交联剂，制备了一种天然水凝胶薄膜，并用于橙皮苷铜（Ⅱ）（NH-Cu（Ⅱ））的控制释放。通过 ATR-FTIR、XRD、TGA 和 DSC 对水凝胶薄膜进行了表征。该薄膜表现出了控制溶胀行为；还在不同的溶液中，包括蒸馏水、不同的盐溶液（包括 0.9%NaCl）和不同 pH 值的溶液，研究了 NH-Cu（Ⅱ）从水凝胶薄膜中的释放行为。噻唑蓝比色法和相对增长率被用来评估所制备的水凝胶薄膜的生物相容性和细胞毒性。结果表明，膨胀动力学遵循菲克扩散和 Schott 二级动力学模型。由于添加了 CNC，水凝胶薄膜的机械性能得到了增强，高温下的热稳定性也得到了提高，而 CNC 的添加量影响了水凝胶薄膜在不同溶液中的溶胀比、盐敏感性和 pH 敏感性。此外，CNC 极大地提高了水凝胶薄膜的载药量和包封效率，最佳 CNC 添加量为 4%，载药量为 753.75mg/g，累积释放率为 85.08%。具有细胞相容性和非细胞毒性的水凝胶薄膜有望用作药物输送和控制释放材料。
