Laboratory of Morphometry, Metabolism, and Cardiovascular Diseases, Biomedical Center, Institute of Biology, The University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
Laboratory of Morphometry, Metabolism, and Cardiovascular Diseases, Biomedical Center, Institute of Biology, The University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
Nutrition. 2019 Sep;65:103-112. doi: 10.1016/j.nut.2019.02.020. Epub 2019 Mar 28.
Intermittent fasting (IF) is a nutritional intervention with significant metabolic effects on the liver that are not yet fully understood. The aim of this study was to investigate the effects of IF on body mass, lipid profile, glucose metabolism, liver lipogenesis, β-oxidation, and inflammation.
We used cellular and molecular techniques to investigate the effects of IF on 3-mo-old male C57 BL/6 mice that were fed control (10% kcal fat), high-fat (HF; 50% kcal fat), or high-fructose (HFr; 50% kcal fructose) diets for 8 wk. Half of the animals were submitted to IF (1 d fed, 1 d fast) for an additional 4 wk.
Although food intake on the fed day did not differ between the groups, mice in the HF and HFr groups showed diminished body mass, total cholesterol, and triacylglycerol levels. Also, plasma adiponectin increased in the HFr group and leptin decreased in the HF mice. Oral glucose tolerance test and insulin were ameliorated by IF, regardless of the diet consumed (HF or HFr), and decreased hepatic lipogenesis and increased β-oxidation markers, resulting in a reduction of the hepatic steatosis and inflammation.
There were beneficial effects of IF even with the continuity of the obesogenic diet and proinflammatory diet in mice. It is recommended that based on the beneficial effects of IF on glucose and liver metabolism and inflammation that IF be a coadjutant factor in the treatment of hepatic metabolic issues and steatosis.
间歇性禁食（IF）是一种对肝脏具有重要代谢影响的营养干预措施，但目前尚未完全了解。本研究旨在探讨 IF 对体重、血脂谱、葡萄糖代谢、肝脏脂肪生成、β-氧化和炎症的影响。
我们使用细胞和分子技术研究了 IF 对 3 个月大的雄性 C57BL/6 小鼠的影响，这些小鼠在 8 周内分别喂食对照（10%卡路里脂肪）、高脂肪（HF；50%卡路里脂肪）或高果糖（HFr；50%卡路里果糖）饮食。一半的动物接受了额外的 4 周 IF（1 天喂食，1 天禁食）。
尽管喂食日的食物摄入量在各组之间没有差异，但 HF 和 HFr 组的小鼠体重、总胆固醇和三酰甘油水平降低。此外，HFr 组的血浆脂联素增加，HF 组的瘦素减少。无论摄入的饮食（HF 或 HFr）如何，IF 均可改善口服葡萄糖耐量试验和胰岛素，降低肝脂肪生成并增加β-氧化标志物，从而减少肝脂肪变性和炎症。
即使在继续给予肥胖和促炎饮食的情况下，IF 也具有有益的效果。建议根据 IF 对葡萄糖和肝脏代谢以及炎症的有益影响，将 IF 作为治疗肝脏代谢问题和脂肪变性的辅助因素。
