Troyanova-Wood Maria, Meng Zhaokai, Yakovlev Vladislav V
Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843-3120, USA.
Department of Physics, Zhejiang University, Hangzhou, Zhejiang 310027, China.
Biomed Opt Express. 2019 Mar 11;10(4):1774-1781. doi: 10.1364/BOE.10.001774. eCollection 2019 Apr 1.
The main objective of the present study is to evaluate the use of Brillouin microspectroscopy for differentiation of melanoma and normal tissues based on elasticity measurements. Previous studies of malignant melanoma show that the lesion is stiffer than the surrounding healthy tissue. We hypothesize that elasticity-specific Brillouin spectroscopy can be used to distinguish between healthy and cancerous regions of an excised melanoma from a Sinclair miniature swine. Brillouin measurements of non-regressing and regressing melanomas and the surrounding healthy tissues were performed. Based on the Brillouin measurements, the melanomas and healthy tissues can be successfully differentiated. The stiffness of both tumors is found to be significantly greater than the healthy tissues. Notably, we found that the elasticity of regressing melanoma is closer to that of the normal tissue. The results indicate that Brillouin spectroscopy can be utilized as a tool for elasticity-based differentiation between malignant melanoma and surrounding healthy tissue, with potential use for melanoma boundary identification, monitoring tumor progression, or response to treatment.
本研究的主要目的是评估基于弹性测量的布里渊显微光谱法在区分黑色素瘤和正常组织方面的应用。先前对恶性黑色素瘤的研究表明，病变比周围健康组织更硬。我们假设，基于弹性的布里渊光谱法可用于区分切除的辛克莱小型猪黑色素瘤的健康区域和癌性区域。对非消退性和消退性黑色素瘤及其周围健康组织进行了布里渊测量。基于布里渊测量，黑色素瘤和健康组织能够成功区分。发现两种肿瘤的硬度均显著高于健康组织。值得注意的是，我们发现消退性黑色素瘤的弹性更接近正常组织。结果表明，布里渊光谱法可作为一种基于弹性区分恶性黑色素瘤和周围健康组织的工具，在黑色素瘤边界识别、监测肿瘤进展或治疗反应方面具有潜在用途。
