Napooni Sara, Delavari Mahdi, Arbabi Mohsen, Barkheh Hossein, Rasti Sima, Hooshyar Hossein, Mostafa Hosseinpour Mashkani S
Department of Medical Parasitology and Mycology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran.
Acta Parasitol. 2019 Jun;64(2):367-375. doi: 10.2478/s11686-019-00054-8. Epub 2019 May 13.
In the current era, cystic echinococcosis (CE), as larval stage of Echinococcus granulosus, is considered as a threat to human health. Scolicidal agents used in the surgery of cysts have different side effects. Therefore, the present study aimed to assess the effects of chitosan nanoparticles containing curcumin (Ch-Cu NPs) on the protoscolices of the hydatid cyst in vitro.
Ch-Cu NPs were synthesized using a simple co-precipitation method and their structural and morphological properties were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), zeta analyzer, and Fourier transform infrared (FT-IR) spectroscopy. Then, the effects of different concentrations of Ch-Cu NPs (0.25, 0.05, 1, 2, and 4 mg/mL) on the fatality rate, and the length and width of protoscolices in different times (5, 10, 20, 30, and 60 min) were investigated. In addition, the SEM technique was used to evaluate the structure of the protoscolices after treatment.
Based on the results, the presence of curcumin on the chitosan nanoparticles was confirmed by FT-IR analysis. Further, XRD analysis approved the crystal structure of chitosan NPs. Furthermore, the highest fatality rate was 68% in 4 mg/mL concentration of Ch-Cu NPs. The length and width of protoscolices decreased based on the high concentrations of Ch-Cu NPs, compared to the control group.
Finally, Ch-Cu NPs expressed good scolicidal activities, which made them suitable to be considered as an anti-protoscolex agent.
在当今时代，作为细粒棘球绦虫幼虫阶段的囊型包虫病（CE）被视为对人类健康的一种威胁。用于囊肿手术的杀头节剂有不同的副作用。因此，本研究旨在评估含姜黄素的壳聚糖纳米颗粒（Ch-Cu NPs）在体外对棘球蚴囊肿原头节的影响。
采用简单的共沉淀法合成Ch-Cu NPs，并通过X射线衍射（XRD）、扫描电子显微镜（SEM）、zeta分析仪和傅里叶变换红外（FT-IR）光谱对其结构和形态特性进行表征。然后，研究不同浓度的Ch-Cu NPs（0.25、0.05、1、2和4mg/mL）在不同时间（5、10、20、30和60分钟）对原头节死亡率、长度和宽度的影响。此外，采用SEM技术评估处理后原头节的结构。
结果表明，通过FT-IR分析证实了壳聚糖纳米颗粒上存在姜黄素。此外，XRD分析证实了壳聚糖纳米颗粒的晶体结构。此外，在4mg/mL浓度的Ch-Cu NPs中，最高死亡率为68%。与对照组相比，高浓度的Ch-Cu NPs使原头节的长度和宽度减小。
最后，Ch-Cu NPs表现出良好的杀头节活性，使其适合被视为一种抗原头节剂。
