姜黄素对二氧化钛纳米颗粒诱导的小鼠睾丸毒性的保护作用。
Protective effects of Curcumin on testicular toxicity induced by titanium dioxide nanoparticles in mice.
作者信息
Karimi Samaneh, Khorsandi Layasadat, Nejaddehbashi Fereshteh
机构信息
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Cell and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
JBRA Assist Reprod. 2019 Oct 14;23(4):344-351. doi: 10.5935/1518-0557.20190031.
OBJECTIVE
In this study, we investigated the preventing effects of Curcumin (Cur) against titanium dioxide nanoparticle (NTiO)-induced mouse testicular damage.
METHODS
We assessed NTiO-intoxicated mice received 50mg/kg of NTiO for 35 days. The Cur + NTiO group was pretreated with Cur (200 mg/kg) for 7 days prior to administering NTiO. Sperm parameters, testosterone concentration, histological criteria, morphometric parameters and Johnsen's scoring.
RESULTS
NTiO significantly reduced testicular weight, testosterone concentration, morphometric parameters, Johnsen's scoring and sperm quality (p<0.01), as well as a significant increase in histological criteria. Pretreatment with Cur reduced testicular weight, ameliorated morphometric parameters, increased Johnsen's scoring, elevated testosterone levels, and increased histological criteria such as vacuolization, detachment, and sloughing of germ cells into the seminiferous tubules. Cur also improved sperm parameters including sperm count, motility, and percentage of abnormality.
CONCLUSION
Cur was found to have a potent protective effect against spermatogenesis defects induced by nanoparticles in mice.
目的
在本研究中，我们调查了姜黄素（Cur）对二氧化钛纳米颗粒（NTiO）诱导的小鼠睾丸损伤的预防作用。
方法
我们评估了接受50mg/kg NTiO处理35天的NTiO中毒小鼠。Cur + NTiO组在给予NTiO之前用Cur（200mg/kg）预处理7天。检测精子参数、睾酮浓度、组织学标准、形态计量学参数和约翰森评分。
结果
NTiO显著降低了睾丸重量、睾酮浓度、形态计量学参数、约翰森评分和精子质量（p<0.01），并显著增加了组织学标准。Cur预处理减轻了睾丸重量，改善了形态计量学参数，提高了约翰森评分，升高了睾酮水平，并增加了组织学标准，如生殖细胞空泡化、脱离和脱落进入生精小管。Cur还改善了精子参数，包括精子数量、活力和异常百分比。
结论
发现Cur对纳米颗粒诱导的小鼠精子发生缺陷具有强大的保护作用。
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