Mahmoudi Reza, Honarmand Zahra, Karbalay-Doust Saied, Jafari-Barmak Mehrzad, Nikseresht Mohsen, Noorafshan Ali
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.
EXCLI J. 2017 Apr 24;16:583-592. doi: 10.17179/excli2017-143. eCollection 2017.
Sodium metabisulfite (Na-MBS) is a disinfectant and preservative agent. Some organ including testicle would be in danger in the case of Na-MBS consumption. Curcumin (CUR) is the constituent of turmeric with protective properties. The effect of CUR on testicles in rats exposed to Na-MBS evaluated using stereological methods. Sprague-Dawley rats were divided into eight groups. The rats in groups I to VIII received the following respectively: distilled water, CUR (100 mg/kg/day), low (0.7 mg/kg/day: acceptable daily intake), intermediate (7 mg/ kg/day), and high (70 mg/kg/day) doses of Na-MBS, and low, intermediate, and high doses of Na-MBS plus CUR. After 7 weeks, the testicles were analyzed. The volume of seminiferous tubule, tubular epithelium and tubule length reduced (25-40 %) on average in the rats that received intermediate and high doses of Na-MBS, while the connective tissue volume increased (15-20 %) in both groups (P<0.01). Besides, 19-36 % and 41-57 % of the cells (spermatogonia types A and B, spermatids, Sertoli and Leydig) were lost in the rats that received intermediate and high doses of Na-MBS respectively in comparison to the control groups. Nonetheless, all the above-mentioned alterations ameliorated drastically in the rats that received Na-MBS plus CUR compared to those exposed to Na-MBS without CUR therapy (P<0.01). The acceptable daily intake of Na-MBS for 7 weeks did not affect on testicular parameters. CUR (100 mg/kg/day) could prevent structural impairments of testicles in the rats induced by Na-MBS (7 and 70 mg/kg/day).
焦亚硫酸钠（Na - MBS）是一种消毒剂和防腐剂。摄入Na - MBS会使包括睾丸在内的一些器官处于危险之中。姜黄素（CUR）是姜黄的成分，具有保护特性。本研究使用体视学方法评估了CUR对暴露于Na - MBS的大鼠睾丸的影响。将Sprague - Dawley大鼠分为八组。第I至VIII组大鼠分别接受以下处理：蒸馏水、CUR（100毫克/千克/天）、低剂量（0.7毫克/千克/天：可接受的每日摄入量）、中剂量（7毫克/千克/天）和高剂量（70毫克/千克/天）的Na - MBS，以及低、中、高剂量的Na - MBS加CUR。7周后，对睾丸进行分析。接受中剂量和高剂量Na - MBS的大鼠，其生精小管体积、小管上皮和小管长度平均减少（25 - 40%），而两组的结缔组织体积均增加（15 - 20%）（P<0.01）。此外，与对照组相比，接受中剂量和高剂量Na - MBS的大鼠分别有19 - 36%和41 - 57%的细胞（A型和B型精原细胞、精子细胞、支持细胞和间质细胞）丢失。然而，与未接受CUR治疗而暴露于Na - MBS的大鼠相比，接受Na - MBS加CUR的大鼠上述所有改变均显著改善（P<0.01）。7周内Na - MBS的可接受每日摄入量对睾丸参数无影响。CUR（100毫克/千克/天）可预防Na - MBS（7和70毫克/千克/天）诱导的大鼠睾丸结构损伤。
