Myers L L, Shoop D S
Am J Vet Res. 1987 Apr;48(4):643-5.
Fecal isolates of enterotoxigenic (44 isolates) and nonenterotoxigenic (25 isolates) Bacteroides fragilis were obtained from diarrheic calves (62 isolates), lambs (2 isolates), and pigs (5 isolates). Using a Microtiter whole-cell agglutination test and gel double-diffusion analysis, the isolates were reacted with nonabsorbed rabbit antisera prepared against 13 isolates of enterotoxigenic B fragilis (ETBF). Isolates of B fragilis were antigenically diverse. Thirty-seven (84%) of the 44 isolates of ETBF comprised 13 serogroups on the basis of reaction in the agglutination test. Fourteen (56%) of the 25 isolates of non-ETBF comprised 4 of the 13 groups. Compared with results of the gel-diffusion test, most isolates had a different agglutination test reaction pattern against the 13 antisera. Isolates of ETBF could not be distinguished from non-ETBF. Antigenic heterogeneity of B fragilis facilitated differentiation of individual isolates, a capability that may be useful in future epidemiologic and virulence studies.
从腹泻的犊牛（62株）、羔羊（2株）和猪（5株）中获得了产肠毒素脆弱拟杆菌（44株）和非产肠毒素脆弱拟杆菌（25株）的粪便分离株。使用微量滴定板全细胞凝集试验和凝胶双向扩散分析，将这些分离株与针对13株产肠毒素脆弱拟杆菌（ETBF）制备的未吸收兔抗血清进行反应。脆弱拟杆菌分离株具有抗原多样性。在凝集试验中，44株ETBF中的37株（84%）属于13个血清群。25株非ETBF中的14株（56%）属于13个群中的4个群。与凝胶扩散试验结果相比，大多数分离株对13种抗血清的凝集试验反应模式不同。ETBF分离株与非ETBF分离株无法区分。脆弱拟杆菌的抗原异质性有助于区分单个分离株，这一能力可能在未来的流行病学和毒力研究中有用。
