萨缪尔利什曼原虫糖缀合物。与萨缪尔佩索阿赫氏鞭毛虫的比较。

Leptomonas samueli glycoconjugates. Comparison with Herpetomonas samuelpessoai.

作者信息

Palatnik C B, Previato J O, Gorin P A, Mendonça-Previato L

出版信息

Comp Biochem Physiol B. 1987;86(3):593-9. doi: 10.1016/0305-0491(87)90454-8.

Abstract

Xylose and mannose are the main manosaccharide components of Herpetomonas samuelpessoai and Leptomonas samueli promastigotes. Variations in the xylose/mannose ratios are related to the age of cultures. Phenol-aqueous extraction disclosed in both species the presence of carbohydrate-containing fractions which were both soluble and insoluble in chloroform/methanol/water (10:10.3). The xylose enriched, uronic acid-containing glycoconjugates of L. samueli were mainly composed of (1----4)-linked xylose units (Methylation-mass spectrometry and 13C NMR), similar to the glucuronoxylan of H. samuelpessoai (Mendonça-Previato et al., 1979 Biochem 18 149-154). SDS-PAGE and sugar composition analysis disclosed similarities between glycoconjugates of H. samuelpessoai and L. samueli, two species dwelling in the same host, therefore an example of convergent adaptation.

摘要

木糖和甘露糖是萨氏赫氏鞭毛虫和萨氏细滴虫前鞭毛体的主要单糖成分。木糖/甘露糖比例的变化与培养物的年龄有关。酚-水提取法在这两个物种中均揭示了存在既溶于又不溶于氯仿/甲醇/水（10:10.3）的含碳水化合物级分。萨氏细滴虫富含木糖、含糖醛酸的糖缀合物主要由（1→4）连接的木糖单元组成（甲基化-质谱和13C NMR），类似于萨氏赫氏鞭毛虫的葡糖醛酸木聚糖（门东萨-普雷维亚托等人，1979年，《生物化学》18卷，第149 - 154页）。SDS - PAGE和糖组成分析揭示了栖息于同一宿主的萨氏赫氏鞭毛虫和萨氏细滴虫糖缀合物之间的相似性，因此是趋同适应的一个例子。