a Department of Intervention, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University , Zhengzhou , Henan , China.
b Innoscience Research SdnBhd , Subang Jaya , Selangor , Malaysia.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3029-3036. doi: 10.1080/21691401.2019.1642902.
Nowadays, the synthesis and characterization of gold nanoparticles (AuNPs) from plant based extracts and effects of their anticancer have concerned an important interest. (MT), a conventional Chinese herbal medicine, has long been used for thousands of years to treat tracheitis, asthma, rheumatism, etc. In this present study, we optimize the reaction of parameters to manage the nanoparticle size, which was categorized by high-resolution transmission electron microscopy (HR-TEM). A different characterization method, for example, UV-visible spectroscopy (UV-vis), fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) were performed to consider the synthesized AuNPs getting from the MT leaf extract. The MT-AuNPs were analyzed for their cytotoxicity property against HepG2 cells by MTT analysis. The apoptosis was evaluated by using reactive oxygen species (ROS), migration assay, mitochondrial membrane potential (MMP) and apoptotic protein expression. Interestingly, the findings of our study observed the cytotoxicity effect of synthesized MT-AuNPs at a concentration of 59.62 ± 4.37 μg after 24 hrs treatment. Apoptosis was induced by the MT-AuNPs with enhanced ROS, changed MMP and inhibit the migration assay. Finally, the apoptosis was confirmed by the considerable up-regulation of Bax, caspase-9 and caspase-3, while the anti-apoptotic protein expressions of Bcl-2 and Bcl-XL were down-regulated. Although, in this studies, we evaluated the characterization, synthesis and anticancer action of gold nanoparticles from MT (MT-AuNPS) helpful for liver cancer therapeutics.
如今，从植物提取物中合成和表征金纳米粒子（AuNPs）及其抗癌作用引起了人们的极大兴趣。(MT)，一种传统的中草药，长期以来一直被用于治疗气管炎、哮喘、风湿等疾病。在本研究中，我们优化了反应参数，以控制纳米粒子的尺寸，这是通过高分辨率透射电子显微镜（HR-TEM）分类的。采用不同的表征方法，如紫外-可见分光光度法（UV-vis）、傅里叶变换红外光谱（FT-IR）和X 射线衍射（XRD），来考虑从 MT 叶提取物中获得的合成 AuNPs。通过 MTT 分析研究了 MT-AuNPs 对 HepG2 细胞的细胞毒性。通过活性氧（ROS）、迁移试验、线粒体膜电位（MMP）和凋亡蛋白表达评估细胞凋亡。有趣的是，我们的研究结果观察到，在 24 小时处理后，浓度为 59.62±4.37μg 的合成 MT-AuNPs 具有细胞毒性作用。MT-AuNPs 通过增强 ROS、改变 MMP 和抑制迁移试验诱导细胞凋亡。最后，通过 Bax、caspase-9 和 caspase-3 的显著上调以及抗凋亡蛋白 Bcl-2 和 Bcl-XL 的下调来证实细胞凋亡。虽然在本研究中，我们评估了 MT 中金纳米粒子（MT-AuNPs）的表征、合成和抗癌作用，这有助于肝癌治疗。
