Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China.
Department of Oral Implantology, Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-Sen University, Guangzhou, China.
Clin Oral Implants Res. 2019 Aug;30(8):760-776. doi: 10.1111/clr.13459. Epub 2019 Jun 5.
Periodontitis and peri-implantitis are oral infectious-inflammatory diseases that share similarities in their pathology and etiology. Our objective was to characterize the single-site subgingival and submucosal microbiomes of implant-rehabilitated, partially dentate Chinese subjects (n = 18) presenting with both periodontitis and peri-implantitis.
Subgingival/submucosal plaque samples were collected from four clinically distinct sites in each subject: peri-implantitis submucosa (DI), periodontal pocket (DT), clinically healthy (unaffected) peri-implant submucosa (HI), and clinically healthy (unaffected) subgingival sulcus (HT). The bacterial microbiota present was analyzed using Illumina MiSeq sequencing.
Twenty-six phyla and 5,726 operational taxonomic units (OTUs, 97% sequence similarity cutoff) were identified. Firmicutes, Proteobacteria, Fusobacteria, Bacteroidetes, Actinobacteria, Synergistetes, TM7, and Spirochaetes comprised 99.6% of the total reads detected. Bacterial communities within the DI, DT, HI, and HT sites shared high levels of taxonomic similarity. Thirty-one "core species" were present in >90% sites, with Streptococcus infantis/mitis/oralis (HMT-070/HMT-071/HMT-638/HMT-677) and Fusobacterium sp. HMT-203/HMT-698 being particularly prevalent and abundant. Beta-diversity analyses (PERMANOVA test, weighted UniFrac) revealed the largest variance in the microbiota was at the subject level (46%), followed by periodontal health status (4%). Differing sets of OTUs were associated with periodontitis and peri-implantitis sites, respectively. This included putative "periodontopathogens," such as Prevotella, Porphyromonas, Tannerella, Bacteroidetes [G-5], and Treponema spp. Interaction network analysis identified several putative patterns underlying dysbiosis in periodontitis/peri-implantitis sites.
Species (OTU) composition of the periodontal and peri-implant microbiota varied widely between subjects. The inter-subject variations in subgingival/submucosal microbiome composition outweighed differences observed between implant vs. tooth sites, or between diseased vs. healthy (unaffected) peri-implant/periodontal sites.
牙周炎和种植体周围炎是口腔感染性炎症性疾病，其病理和病因具有相似性。我们的目的是描述患有牙周炎和种植体周围炎的中国部分牙列缺损患者（n=18）单个种植体部位的龈下和黏膜下微生物组。
从每位患者的四个临床不同部位采集龈下/黏膜下斑块样本：种植体周围炎黏膜下（DI）、牙周袋（DT）、临床健康（未受影响）的种植体黏膜下（HI）和临床健康（未受影响）的龈下沟（HT）。使用 Illumina MiSeq 测序分析存在的细菌微生物组。
鉴定出 26 个门和 5726 个操作分类单元（OTU，97%序列相似性截止值）。厚壁菌门、变形菌门、梭杆菌门、拟杆菌门、放线菌门、互养菌门、TM7 和螺旋体门构成了总读数的 99.6%。DI、DT、HI 和 HT 部位的细菌群落具有高度的分类相似性。31 种“核心种”存在于>90%的部位，其中婴儿链球菌/中间链球菌/口腔链球菌（HMT-070/HMT-071/HMT-638/HMT-677）和梭杆菌属 HMT-203/HMT-698 特别普遍和丰富。β多样性分析（PERMANOVA 检验，加权 UniFrac）显示，微生物组的最大变异发生在个体水平（46%），其次是牙周健康状况（4%）。分别与牙周炎和种植体周围炎部位相关的是不同的 OTU 集。这包括推定的“牙周病原体”，如普雷沃菌属、卟啉单胞菌属、丹毒丝菌属、拟杆菌门[G-5]和密螺旋体属。交互网络分析确定了在牙周炎/种植体周围炎部位发生生态失调的几种潜在模式。
牙周和种植体周围微生物群的物种（OTU）组成在个体之间差异很大。龈下/黏膜下微生物组组成的个体间差异大于种植体与牙齿部位之间或疾病与健康（未受影响）种植体/牙周部位之间的差异。
