Biodesign Institute, Arizona State Tempe, AZ, USA; School of Biological and Health Systems Engineering, Arizona State Tempe, AZ, USA; Mayo Clinic College of Medicine and Science, Phoenix, AZ, USA.
European Molecular Biology Laboratory (EMBL) Barcelona, Dr. Aiguader, 88, 08003, Barcelona, Spain.
Curr Opin Chem Biol. 2019 Oct;52:9-15. doi: 10.1016/j.cbpa.2019.04.006. Epub 2019 May 15.
Synthetic biology offers a bottom-up engineering approach that intends to understand complex systems via design-build-test cycles. Embryonic development comprises complex processes that originate at the level of gene regulatory networks in a cell and emerge into collective cellular behaviors with multicellular forms and functions. Here, we review synthetic biology approaches to development that involve building de novo developmental trajectories or engineering control in stem cell-derived multicellular systems. The field of synthetic developmental biology is rapidly growing with the help of recent advances in artificial gene circuits, self-organizing organoids, and controllable tissue microenvironments. The outcome will be a blueprint to decode principles of morphogenesis and to create programmable organoids with novel designs or improved functions.
合成生物学提供了一种自下而上的工程方法，旨在通过设计-构建-测试循环来理解复杂系统。胚胎发育包含复杂的过程，这些过程起源于细胞中的基因调控网络水平，并以多细胞形式和功能的集体细胞行为出现。在这里，我们回顾了涉及从头构建发育轨迹或在干细胞衍生的多细胞系统中工程控制的发育的合成生物学方法。在人工基因电路、自我组织类器官和可控组织微环境等最新进展的帮助下，合成发育生物学领域正在迅速发展。其结果将是一个解码形态发生原理的蓝图，并创建具有新颖设计或改进功能的可编程类器官。
