新型合成生物学方法在发育系统中的应用。
Novel synthetic biology approaches for developmental systems.
机构信息
Developmental Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany.
Eli and Edythe Broad CIRM Center, Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA; Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA.
出版信息
Stem Cell Reports. 2021 May 11;16(5):1051-1064. doi: 10.1016/j.stemcr.2021.04.007.
Recently, developmental systems are investigated with increasing technological power. Still, open questions remain, especially concerning self-organization capacity and its control. Here, we present three areas where synthetic biology tools are used in top-down and bottom-up approaches for studying and constructing developmental systems. First, we discuss how synthetic biology tools can improve stem cell-based organoid models. Second, we discuss recent studies employing user-defined perturbations to study embryonic patterning in model species. Third, we present "toy models" of patterning and morphogenesis using synthetic genetic circuits in non-developmental systems. Finally, we discuss how these tools and approaches can specifically benefit the field of embryo models.
近年来，随着技术能力的不断提升，人们对发展系统进行了深入研究。但是，仍然存在一些悬而未决的问题，尤其是在自我组织能力及其控制方面。在这里，我们介绍了三个领域，在这些领域中，合成生物学工具被用于自上而下和自下而上的方法来研究和构建发展系统。首先，我们讨论了合成生物学工具如何改进基于干细胞的类器官模型。其次，我们讨论了最近利用用户定义的干扰来研究模型物种胚胎模式形成的研究。第三，我们介绍了使用合成遗传回路在非发育系统中进行模式形成和形态发生的“玩具模型”。最后，我们讨论了这些工具和方法如何能够特别有益于胚胎模型领域。
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