t(2;8)染色体易位的κ基因中的N片段插入和区域定向体细胞超突变。
N segment insertion and region-directed somatic hypermutation in a kappa gene of a t(2;8) chromosomal translocation.
作者信息
Klobeck H G, Combriato G, Zachau H G
出版信息
Nucleic Acids Res. 1987 Jun 25;15(12):4877-88. doi: 10.1093/nar/15.12.4877.
Abstract
A detailed molecular analysis of both reciprocal recombination products of the variant t(2;8) chromosomal translocation of the Burkitt lymphoma derived cell line JI and their germline counterparts was carried out. The breakpoint on chromosome 8 is localized 28 kb to the 3' side of the c-myc protooncogene, the breakpoint on chromosome 2 was found to be within an aberrantly rearranged VK gene (abbreviations ref. 1). Novel features of the immunoglobulin moiety involved in this process include insertion of extra nucleotides in the V-J junction which have the characteristics of a N segment as it has been found up to now only in heavy chain and T cell receptor genes; the occurrence of somatic mutations in 8q+ and not in 2p-. These data allow a reconstruction of the course of events in the cell line JI; remarkable sequence regularities at the chromosomal breakpoints consisting of symmetrically placed dinucleotides and elements related to the hepta- and nonanucleotide recombinase recognition sequences are discussed in the context of the translocation mechanism.
摘要
对源自伯基特淋巴瘤的细胞系JI的变异型t(2;8)染色体易位的两个相互重组产物及其种系对应物进行了详细的分子分析。8号染色体上的断点位于原癌基因c-myc 3'端28 kb处，2号染色体上的断点位于一个异常重排的VK基因内（参考文献1）。参与此过程的免疫球蛋白部分的新特征包括在V-J连接中插入额外的核苷酸，这些核苷酸具有N片段的特征，因为到目前为止仅在重链和T细胞受体基因中发现；8q+发生体细胞突变而2p-未发生。这些数据有助于重建细胞系JI中的事件过程；在易位机制的背景下讨论了染色体断点处由对称排列的二核苷酸和与七核苷酸及九核苷酸重组酶识别序列相关的元件组成的显著序列规律。
相似文献
1
N segment insertion and region-directed somatic hypermutation in a kappa gene of a t(2;8) chromosomal translocation.t(2;8)染色体易位的κ基因中的N片段插入和区域定向体细胞超突变。
Nucleic Acids Res. 1987 Jun 25;15(12):4877-88. doi: 10.1093/nar/15.12.4877.
2
The t(8;14) chromosome translocation of the Burkitt lymphoma cell line Daudi occurred during immunoglobulin gene rearrangement and involved the heavy chain diversity region.伯基特淋巴瘤细胞系Daudi的t(8;14)染色体易位发生于免疫球蛋白基因重排过程中，且涉及重链多样性区域。
Proc Natl Acad Sci U S A. 1987 Oct;84(19):6835-9. doi: 10.1073/pnas.84.19.6835.
3
Generation of a variant t(2;8) translocation of Burkitt's lymphoma by site-specific recombination via the kappa light-chain joining signals.通过κ轻链连接信号的位点特异性重组产生伯基特淋巴瘤的变异型t(2;8)易位。
Mol Cell Biol. 1987 Jun;7(6):2037-45. doi: 10.1128/mcb.7.6.2037-2045.1987.
4
The t(8; 14) chromosomal translocation occurring in B-cell malignancies results from mistakes in V-D-J joining.发生在B细胞恶性肿瘤中的t(8; 14)染色体易位是由V-D-J连接错误导致的。
Nature. 1986;324(6093):158-61. doi: 10.1038/324158a0.
5
Translocation of an immunoglobulin kappa locus to a region 3' of an unrearranged c-myc oncogene enhances c-myc transcription.免疫球蛋白κ基因座易位至未重排的c-myc癌基因3'端区域可增强c-myc转录。
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7581-5. doi: 10.1073/pnas.80.24.7581.
6
Variant translocations in sporadic Burkitt's lymphoma detected in fresh tumour material: analysis of three cases.在新鲜肿瘤组织中检测到的散发性伯基特淋巴瘤中的变异易位：三例分析
Br J Haematol. 2000 Sep;110(3):537-46. doi: 10.1046/j.1365-2141.2000.02241.x.
7
c-myc and immunoglobulin kappa light chain constant genes are on the 8q+ chromosome of three Burkitt lymphoma lines with t(2;8) translocations.c-myc和免疫球蛋白κ轻链恒定基因位于三条带有t(2;8)易位的伯基特淋巴瘤细胞系的8q+染色体上。
EMBO J. 1984 Dec 1;3(12):2951-5. doi: 10.1002/j.1460-2075.1984.tb02239.x.
8
Variant translocation of the BCL6 gene to immunoglobulin kappa light chain gene in B-cell lymphoma.B细胞淋巴瘤中BCL6基因向免疫球蛋白κ轻链基因的变异易位。
Jpn J Cancer Res. 1994 Sep;85(9):911-7. doi: 10.1111/j.1349-7006.1994.tb02968.x.
9
Molecular analysis of the t(8;14)(q24;q11) chromosomal breakpoint junctions in the T-cell leukemia line MOLT-16.T细胞白血病细胞系MOLT-16中t(8;14)(q24;q11)染色体断裂点连接的分子分析。
Genes Chromosomes Cancer. 1997 Dec;20(4):363-71. doi: 10.1002/(sici)1098-2264(199712)20:4<363::aid-gcc7>3.0.co;2-#.
10
Regulatory elements in the immunoglobulin kappa locus induce c-myc activation in Burkitt's lymphoma cells.免疫球蛋白κ轻链基因座中的调控元件在伯基特淋巴瘤细胞中诱导c-myc激活。
Curr Top Microbiol Immunol. 1995;194:415-22. doi: 10.1007/978-3-642-79275-5_48.
引用本文的文献
1
AID-targeting and hypermutation of non-immunoglobulin genes does not correlate with proximity to immunoglobulin genes in germinal center B cells.AID 靶向和非免疫球蛋白基因的超突变与生发中心 B 细胞中免疫球蛋白基因的邻近性无关。
PLoS One. 2012;7(6):e39601. doi: 10.1371/journal.pone.0039601. Epub 2012 Jun 29.
2
Frequent occurrence of deletions and duplications during somatic hypermutation: implications for oncogene translocations and heavy chain disease.体细胞高频突变过程中缺失和重复的频繁发生：对癌基因易位和重链病的影响
Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2463-8. doi: 10.1073/pnas.95.5.2463.
3
c-myc activation renders proliferation of Epstein-Barr virus (EBV)-transformed cells independent of EBV nuclear antigen 2 and latent membrane protein 1.c-myc激活使爱泼斯坦-巴尔病毒（EBV）转化的细胞增殖独立于EBV核抗原2和潜伏膜蛋白1。
Proc Natl Acad Sci U S A. 1996 Sep 17;93(19):10411-6. doi: 10.1073/pnas.93.19.10411.
4
Regulatory elements in the immunoglobulin kappa locus induce c-myc activation and the promoter shift in Burkitt's lymphoma cells.免疫球蛋白κ轻链基因座中的调控元件可诱导伯基特淋巴瘤细胞中的c-myc激活及启动子转换。
EMBO J. 1993 Oct;12(10):3913-20. doi: 10.1002/j.1460-2075.1993.tb06069.x.
5
Evidence of antigen receptor-influenced oligoclonal B lymphocyte expansion in the synovium of a patient with longstanding rheumatoid arthritis.一名长期类风湿性关节炎患者滑膜中存在抗原受体影响的寡克隆B淋巴细胞扩增的证据。
J Clin Invest. 1994 Jan;93(1):361-70. doi: 10.1172/JCI116968.
6
Alternative V kappa gene rearrangements in a murine B cell lymphoma. An explantation for idiotypic heterogeneity.小鼠B细胞淋巴瘤中的替代性Vκ基因重排。独特型异质性的一种解释。
J Exp Med. 1988 Nov 1;168(5):1607-20. doi: 10.1084/jem.168.5.1607.
7
Physical map of the human immunoglobulin K locus and its implications for the mechanisms of VK-JK rearrangement.人类免疫球蛋白K基因座的物理图谱及其对VK-JK重排机制的影响。
Nucleic Acids Res. 1987 Dec 10;15(23):9667-76. doi: 10.1093/nar/15.23.9667.
8
Linking of the human immunoglobulin VK and JKCK regions by chromosomal walking.通过染色体步移连接人类免疫球蛋白VK和JK基因座的恒定区。
Nucleic Acids Res. 1987 Dec 10;15(23):9655-65. doi: 10.1093/nar/15.23.9655.
9
Three breakpoints of variant t(2;8) translocations in Burkitt's lymphoma cells fall within a region 140 kilobases distal from c-myc.伯基特淋巴瘤细胞中变异型t(2;8)易位的三个断点位于距c-myc基因140千碱基远的一个区域内。
Mol Cell Biol. 1989 May;9(5):2105-13. doi: 10.1128/mcb.9.5.2105-2113.1989.
10
Short, direct repeats at the breakpoints of deletions of the retinoblastoma gene.视网膜母细胞瘤基因缺失断点处的短而直接的重复序列。
Proc Natl Acad Sci U S A. 1989 Jul;86(13):5044-8. doi: 10.1073/pnas.86.13.5044.
本文引用的文献
1
Variant (6 ; 15) translocation in a murine plasmacytoma occurs near an immunoglobulin kappa gene but far from the myc oncogene.小鼠浆细胞瘤中的（6；15）易位变体发生在免疫球蛋白κ基因附近，但远离myc癌基因。
Nature. 1984;312(5996):777-9. doi: 10.1038/312777a0.
2
Translocation of an immunoglobulin kappa locus to a region 3' of an unrearranged c-myc oncogene enhances c-myc transcription.免疫球蛋白κ基因座易位至未重排的c-myc癌基因3'端区域可增强c-myc转录。
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7581-5. doi: 10.1073/pnas.80.24.7581.
3
A variant translocation places the lambda immunoglobulin genes 3' to the c-myc oncogene in Burkitt's lymphoma.在伯基特淋巴瘤中，一种变异易位将λ免疫球蛋白基因置于c-myc癌基因的3'端。
Nature. 1984;307(5953):752-5. doi: 10.1038/307752a0.
4
Novel kappa light-chain gene rearrangements in mouse lambda light chain-producing B lymphocytes.小鼠产生λ轻链的B淋巴细胞中的新型κ轻链基因重排。
Nature. 1984;307(5953):749-52. doi: 10.1038/307749a0.
5
A novel type of aberrant recombination in immunoglobulin genes and its implications for V-J joining mechanism.免疫球蛋白基因中一种新型的异常重组及其对V-J连接机制的影响。
Nature. 1983;302(5905):260-3. doi: 10.1038/302260a0.
6
Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.利用寡脱氧核苷酸定向诱变构建改良的M13载体。
Gene. 1983 Dec;26(1):101-6. doi: 10.1016/0378-1119(83)90040-9.
7
Lambda replacement vectors carrying polylinker sequences.携带多克隆位点序列的λ置换载体。
J Mol Biol. 1983 Nov 15;170(4):827-42. doi: 10.1016/s0022-2836(83)80190-9.
8
Somatic generation of antibody diversity.抗体多样性的体细胞产生。
Nature. 1983 Apr 14;302(5909):575-81. doi: 10.1038/302575a0.
9
Evolution of human immunoglobulin kappa J region genes.人类免疫球蛋白κ J 区基因的进化
J Biol Chem. 1982 Feb 10;257(3):1516-22.
10
Differences between germ-line and rearranged immunoglobulin V kappa coding sequences suggest a localized mutation mechanism.种系免疫球蛋白Vκ编码序列与重排免疫球蛋白Vκ编码序列之间的差异提示了一种局部突变机制。
Nature. 1981 Jun 25;291(5817):668-70. doi: 10.1038/291668a0.
Zotero 插件