Erikson J, Nishikura K, ar-Rushdi A, Finan J, Emanuel B, Lenoir G, Nowell P C, Croce C M
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7581-5. doi: 10.1073/pnas.80.24.7581.
We have studied somatic cell hybrids between mouse myeloma and JI Burkitt lymphoma cells carrying a t(2;8) chromosome translocation for the expression of human kappa chains. and for the presence and rearrangements of the human c-myc oncogene and kappa chain genes. Our results indicate that the c-myc oncogene is unrearranged and remains on the 8q+ chromosome of JI cells. Two rearranged C kappa genes were detected: the expressed allele on normal chromosome 2 and the excluded kappa allele that was translocated from chromosome 2 to the involved chromosome 8 (8q+). The distribution of V kappa and C kappa genes in hybrid clones retaining different human chromosomes indicated that C kappa is distal to V kappa on 2p and that the breakpoint in this Burkitt lymphoma is within the region carrying V kappa genes. High levels of transcripts of the c-myc gene were found when it resided on the 8q+ chromosome but not on the normal chromosome 8, demonstrating that translocation of a kappa locus to region distal to the c-myc oncogene enhances c-myc transcription.
我们研究了小鼠骨髓瘤细胞与携带t(2;8)染色体易位的JI伯基特淋巴瘤细胞之间的体细胞杂种，以检测人类κ链的表达，以及人类c-myc癌基因和κ链基因的存在与重排情况。我们的结果表明，c-myc癌基因未发生重排，仍位于JI细胞的8q+染色体上。检测到两个重排的Cκ基因：正常2号染色体上表达的等位基因，以及从2号染色体易位至受累的8号染色体（8q+）上的排除κ等位基因。在保留不同人类染色体的杂种克隆中Vκ和Cκ基因的分布表明，Cκ在2p上位于Vκ的远端，并且该伯基特淋巴瘤中的断点位于携带Vκ基因的区域内。当c-myc基因位于8q+染色体上而非正常8号染色体上时，可发现其高水平转录本，这表明κ基因座易位至c-myc癌基因远端区域可增强c-myc转录。
