Siddika Mst Ayesha, Oi Hiroki, Hidaka Kumi, Sugiyama Hiroshi, Endo Masayuki, Matsumura Shigeyoshi, Ikawa Yoshiya
Graduate School of Innovative Life Science, University of Toyama, Toyama 930-8555, Toyama, Japan.
Department of Chemistry, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Toyama, Japan.
Molecules. 2023 Sep 6;28(18):6465. doi: 10.3390/molecules28186465.
The multimolecular assembly of three-dimensionally structured proteins forms their quaternary structures, some of which have high geometric symmetry. The size and complexity of protein quaternary structures often increase in a hierarchical manner, with simpler, smaller structures serving as units for larger quaternary structures. In this study, we exploited oligomerization of a ribozyme cyclic trimer to achieve larger ribozyme-based RNA assembly. By installing kissing loop (KL) interacting units to one-, two-, or three-unit RNA molecules in the ribozyme trimer, we constructed dimers, open-chain oligomers, and branched oligomers of ribozyme trimer units. One type of open-chain oligomer preferentially formed a closed tetramer containing 12 component RNAs to provide 12 ribozyme units. We also observed large assembly of ribozyme trimers, which reached 1000 nm in size.
三维结构蛋白质的多分子组装形成其四级结构，其中一些具有高度的几何对称性。蛋白质四级结构的大小和复杂性通常以分级方式增加，较简单、较小的结构作为较大四级结构的单元。在本研究中，我们利用核酶环状三聚体的寡聚化来实现更大的基于核酶的RNA组装。通过在核酶三聚体中的单、二或三单元RNA分子上安装亲吻环（KL）相互作用单元，我们构建了核酶三聚体单元的二聚体、开链寡聚体和分支寡聚体。一种开链寡聚体优先形成含有12个组分RNA的封闭四聚体，以提供12个核酶单元。我们还观察到核酶三聚体的大型组装，其大小达到1000纳米。
