Minsakorn Sutthida, Nuplod Khwannapa, Puttarak Panupong, Chawengkirttikul Runglawan, Panyarachun Busaba, Ngamniyom Arin, Charoenkul Tanapat, Jaisa-Aad Methasit, Panyarachun Panadda, Anuracpreeda Panat
Faculty of Veterinary Technology, Kasetsart University, Juttajak, Bangkok, 10900, Thailand.
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla, 90112, Thailand.
Acta Parasitol. 2019 Sep;64(3):566-574. doi: 10.2478/s11686-019-00072-6. Epub 2019 May 20.
Paramphistomosis is a disease caused by the rumen flukes which cause an acute gastroenteritis and anemia with high mortality particularly in young ruminants.
In this study, we have investigated the anthelmintic effect of medicinal plant extracts from leaves and heartwoods of Cassia siamea L., roots of Plumbago zeylanica L. and Plumbago indica L., and leaves of Terminalia catappa L. against Carmyerius spatiosus.
The highest anthelminthic effect on the flukes after 24 h of exposure was found in heartwood ethyl acetate extract of C. siamea (LC = 374.30; LC = 749.03 ppm), root n-butanol extract of P. zeylanica (LC = 1005.12; LC = 2411.55 ppm), root hexane, ethyl acetate, and n-butanol extract of P. indica (LC = 34.38, 211.34, 506.92; LC = 64.09, 496.05, 934.86 ppm), and leaf n-butanol and water extract of T. catappa (LC = 487.17, 470.28; LC = 913.27, 848.23 ppm). When observed by scanning electron microscopy, the tegument showed similar sequence of morphological changes after treatments with all plant extracts, comprising of swelling of ridges and folds, blebbing, rupturing of the blebs, erosion, lesion and disruption of the tegument.
This study is the first report on the anthelmintic activity of plant extracts to C. spatiosus; therefore, these plant extracts are highly effective in the elimination of adult rumen flukes.
双口吸虫病是由瘤胃吸虫引起的一种疾病，可导致急性肠胃炎和贫血，在幼龄反刍动物中死亡率很高。
在本研究中，我们研究了铁刀木的叶和心材、白花丹和印度白花丹的根以及榄仁树的叶的药用植物提取物对阔盘吸虫的驱虫效果。
暴露24小时后，对吸虫驱虫效果最佳的是铁刀木心材乙酸乙酯提取物（LC = 374.30；LC = 749.03 ppm）、白花丹根正丁醇提取物（LC = 1005.12；LC = 2411.55 ppm）、印度白花丹根己烷、乙酸乙酯和正丁醇提取物（LC = 34.38、211.34、506.92；LC = 64.09、496.05、934.86 ppm）以及榄仁树叶正丁醇和水提取物（LC = 487.17、470.28；LC = 913.27、848.23 ppm）。通过扫描电子显微镜观察发现，用所有植物提取物处理后，虫体体表呈现出相似的形态变化顺序，包括嵴和褶皱肿胀、起泡、泡破裂、侵蚀、损伤和体表破坏。
本研究是关于植物提取物对阔盘吸虫驱虫活性的首次报道；因此，这些植物提取物在消除成年瘤胃吸虫方面非常有效。
