Lin Bei-Bei, Liu Xiang, Wu San-Qiao, Zheng Hong-Xing, Huo Ke-Ke, Qi Shan-Shan, Chen Chen
Chinese-German Joint Laboratory for Natural Product Research, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, 723000, Shaanxi, P. R. China.
Vitamin D research institute, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, 723000, Shaanxi, P. R. China.
Chem Biodivers. 2019 Jul;16(7):e1900080. doi: 10.1002/cbdv.201900080. Epub 2019 Jun 21.
The objective of this study is to compare the efficacy of ethanol extracts from different parts of Sophora viciifolia. The content of polyphenols, flavonoids, alkaloids, and antioxidant capacity, antimicrobial activity were investigated, and individual polyphenols and alkaloids were analyzed and quantified by ultra-high performance liquid chromatography (UPLC). The microdilution method was used to determine the antimicrobial activity of extracts from S. viciifolia on six strains. The results for extracts from the different parts (flowers, leaves, and fruit) were compared in varying concentrations to determine whether one extract source is superior to another. Testing verified that extracts from the different parts of S. viciifolia did vary, as expected. For example, extract from the leaves had the best antimicrobial activity against pathogenic Candida albicans, but all extracts had good antimicrobial activity against the six tested strains. These results reveal that the active substances in S. viciifolia are abundant and have good antioxidant and antimicrobial activities, which can provide theoretical support for the subsequent development and utilization of S. viciifolia extracts.
本研究的目的是比较苦豆子不同部位乙醇提取物的功效。对多酚、黄酮、生物碱的含量以及抗氧化能力、抗菌活性进行了研究,并通过超高效液相色谱(UPLC)对单个多酚和生物碱进行了分析和定量。采用微量稀释法测定苦豆子提取物对六种菌株的抗菌活性。比较了不同部位(花、叶和果实)提取物在不同浓度下的结果,以确定一种提取物来源是否优于另一种。测试证实,苦豆子不同部位的提取物确实如预期的那样存在差异。例如,叶提取物对致病性白色念珠菌具有最佳抗菌活性,但所有提取物对六种测试菌株均具有良好的抗菌活性。这些结果表明,苦豆子中的活性物质丰富,具有良好的抗氧化和抗菌活性,可为苦豆子提取物的后续开发利用提供理论支持。