Primary structure of soybean lipoxygenase-1.

The primary structure of lipoxygenase-1 from soybeans has been determined. The results were deduced from the nucleotide sequence of the complete cDNA for isozyme lipoxygenase-1 and were confirmed by Edman sequencing of selected peptides obtained by CNBr cleavage and supported by carboxyl-terminal sequencing of the intact protein and by partial peptide analysis employing fast atom bombardment-mass spectroscopy. The enzyme has a molecular weight of 94,038 based on its content of 838 amino acids.