Department of Ecological and Biological Sciences, University of Tuscia, 01100 Viterbo, Italy.
Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 35131 Padova, Italy.
Int J Mol Sci. 2019 May 22;20(10):2525. doi: 10.3390/ijms20102525.
The role of jasmonates in defense priming has been widely recognized. Priming is a physiological process by which a plant exposed to low doses of biotic or abiotic elicitors activates faster and/or stronger defense responses when subsequently challenged by a stress. In this work, we investigated the impact of MeJA-induced defense responses to mechanical wounding in rice (). The proteome reprogramming of plants treated with MeJA, wounding or MeJA+wounding has been in-depth analyzed by using a combination of high throughput profiling techniques and bioinformatics tools. Gene Ontology analysis identified protein classes as defense/immunity proteins, hydrolases and oxidoreductases differentially enriched by the three treatments, although with different amplitude. Remarkably, proteins involved in photosynthesis or oxidative stress were significantly affected upon wounding in MeJA-primed plants. Although these identified proteins had been previously shown to play a role in defense responses, our study revealed that they are specifically associated with MeJA-priming. Additionally, we also showed that at the phenotypic level MeJA protects plants from oxidative stress and photosynthetic damage induced by wounding. Taken together, our results add novel insight into the molecular actors and physiological mechanisms orchestrated by MeJA in enhancing rice plants defenses after wounding.
茉莉酸在防御激发中的作用已得到广泛认可。激发是一种生理过程，植物暴露于低剂量的生物或非生物激发子后，当随后受到胁迫时，会更快和/或更强地激活防御反应。在这项工作中，我们研究了茉莉酸诱导的防御反应对水稻机械损伤的影响（）。通过使用高通量分析技术和生物信息学工具的组合，深入分析了用茉莉酸、损伤或茉莉酸+损伤处理的植物的蛋白质组重编程。基因本体分析确定了防御/免疫蛋白、水解酶和氧化还原酶等蛋白类，这三种处理均有差异富集，但幅度不同。值得注意的是，在茉莉酸预处理的植物中，与光合作用或氧化应激相关的蛋白质在损伤时受到显著影响。尽管这些已鉴定的蛋白质先前被证明在防御反应中起作用，但我们的研究表明它们与茉莉酸激发特异性相关。此外，我们还表明，在表型水平上，茉莉酸可以保护植物免受损伤引起的氧化应激和光合作用损伤。总之，我们的研究结果为茉莉酸在增强水稻植物防御能力方面的分子作用和生理机制提供了新的见解。
