钩吻素甲和钩吻素乙对 PC12 细胞的细胞毒性作用差异。

College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.

Hunan Engineering Technology Research Center of Veterinary Drugs, Hunan Agricultural University, Changsha 410128, China.

Molecules. 2019 May 25;24(10):2004. doi: 10.3390/molecules24102004.

Two optical isomers, +/- gelsemine (1, 2), together with one known compound were isolated from the whole plant of . The structures of the separated constituents were elucidated on 1D and 2D (H-H COSY, HMBC, HSQC) NMR spectroscopy and high-resolution mass spectrometry (HRMS). The isolated alkaloids were tested in vitro for cytotoxic potential against PC12 cells by the MTT assay. As a result, (+) gelsemine (compound ) exhibited cytotoxic activity against PC12 cells with an IC value of 31.59 μM, while (-) gelsemine (compound ) was not cytotoxic.

从全株中分离得到两种光学异构体，+/-钩藤碱（1、2）和一种已知化合物。通过 1D 和 2D（H-H COSY、HMBC、HSQC）NMR 光谱和高分辨率质谱（HRMS）解析了分离成分的结构。通过 MTT 法测定了分离得到的生物碱对 PC12 细胞的体外细胞毒性。结果表明，（+）钩藤碱（化合物）对 PC12 细胞具有细胞毒性，IC 值为 31.59 μM，而（-）钩藤碱（化合物）则没有细胞毒性。