Kraaijeveld C A, Harmsen M, Khader Boutahar-Trouw B
Infect Immun. 1979 Feb;23(2):213-8. doi: 10.1128/iai.23.2.213-218.1979.
Intracutaneous immunization of BALB/c mice with purified inactivated Semliki Forest virus resulted in cellular immunity without detectable antibodies. The animals were protected against subcutaneous challenge, from which the challenge virus spreads slowly. After intraperitoneal challenge, which permits a rapid virus spread, the protection was marginal. Stimulation of the intraperitoneal cell population with thioglycolate before challenge resulted in complete protection. The protection could be transferred to normal mice with peripheral lymph node cells, but not with spleen cells. The course of the infection in immunized and normal mice was also studied. Semliki Forest virus does not multiply in peritoneal cells in vivo. In immunized mice part of the challenge virus in the peritoneal cavity was rapidly eliminated and viremia was reduced. After challenge, immunized mice produced less antibody than normal mice.
用纯化的灭活塞姆利基森林病毒对BALB/c小鼠进行皮内免疫，可诱导出细胞免疫，且未检测到抗体。这些动物受到保护，可抵御皮下攻击，皮下攻击的病毒传播缓慢。腹腔内攻击时，病毒传播迅速，保护作用微弱。攻击前用巯基乙酸盐刺激腹腔细胞群体可实现完全保护。这种保护作用可通过外周淋巴结细胞转移给正常小鼠，但不能通过脾细胞转移。还研究了免疫小鼠和正常小鼠的感染过程。塞姆利基森林病毒在体内不会在腹腔细胞中增殖。在免疫小鼠中，腹腔内的部分攻击病毒被迅速清除，病毒血症减轻。攻击后，免疫小鼠产生的抗体比正常小鼠少。
