van der Groen G, Vanden Berghe D A, Pattyn S R
J Gen Virol. 1977 Feb;34(2):353-61. doi: 10.1099/0022-1317-34-2-353.
In vitro cultured mouse peritoneal macrophages are inefficient host cells for both alpha and flaviruses tested. Production of infectious virus ceased 6 to 24 h after infection, except for virulent and avirulent SF strains. This limited growth is unrelated to interferon production. No correlation was found between the LD50 of different virus strains for mice and the virus yields of in vitro infected macrophages therefrom. When macrophages from SF immunized mice were inoculated in vitro with SF or EEE, a cytopathic effect occurred, while the multiplication of SF, but not of EEE, was decreased. Virus multiplication in proteose peptone induced macrophages was enhanced for SF strains only. These results are discussed in relation to the virulence of several arboviruses for mice.
体外培养的小鼠腹腔巨噬细胞对于所测试的甲型病毒和黄病毒而言，都是低效的宿主细胞。感染后6至24小时，传染性病毒的产生停止，但强毒株和无毒力的SF毒株除外。这种有限的生长与干扰素的产生无关。不同病毒株对小鼠的半数致死剂量（LD50）与由此产生的体外感染巨噬细胞的病毒产量之间未发现相关性。当用SF或EEE体外接种来自经SF免疫小鼠的巨噬细胞时，会出现细胞病变效应，而SF的增殖减少，但EEE没有。仅对于SF毒株，蛋白胨诱导的巨噬细胞中的病毒增殖增强。结合几种虫媒病毒对小鼠的毒力对这些结果进行了讨论。
