Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de Mexico, AP 70228, Ciudad de Mexico CP 04510, Mexico.
Laboratorio de Genotoxicología y Mutagénesis Ambientales, Departamento de Ciencias Ambientales, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de Mexico, Ciudad de Mexico CP 04510, Mexico.
Int J Environ Res Public Health. 2019 May 20;16(10):1784. doi: 10.3390/ijerph16101784.
Bisphenol A (BPA) is an endocrine disruptor of estrogenic nature. During the early stages of development, any exposure to BPA can have long-term effects. In this work, we study the potential alterations to the humoral antitumor immune (IgM) response in adult life after a single neonatal exposure to BPA. Female syngeneic BALB/c mice were exposed to a single dose of BPA of 250 μg/kg. Once sexual maturity was reached, a breast tumor was induced. After 25 days, the serum was obtained, and the populations of B cells in the spleen and lymph nodes were analyzed by flow cytometry. The reactivity of IgM was evaluated by 2D immunoblots. No significant changes were found in the B cell populations in the peripheral lymph nodes and the spleen. The level of ERα expression was not significantly different. However, the IgM reactivity was affected. In individuals treated with BPA, a decrease in the number of IgMs that recognize tumor antigens was observed. The possibility that these antibodies are the high affinity products of the adaptive response is discussed. The recognition of IgG was also evaluated but a null recognition was found in the controls as in the individuals treated with the 4T1 cells.
双酚 A（BPA）是一种具有雌激素性质的内分泌干扰物。在发育的早期阶段，任何接触 BPA 的情况都可能产生长期影响。在这项工作中，我们研究了在单一新生期接触 BPA 后，对成年期体液抗肿瘤免疫（IgM）反应的潜在改变。雌性同基因 BALB/c 小鼠接受了 250μg/kg 的单次 BPA 暴露。一旦达到性成熟，就会诱导乳腺癌。25 天后，获得血清，并通过流式细胞术分析脾和淋巴结中 B 细胞的群体。通过 2D 免疫印迹评估 IgM 的反应性。在外周淋巴结和脾脏中的 B 细胞群体中未发现明显变化。ERα 表达水平没有显著差异。但是，IgM 反应性受到影响。在接受 BPA 治疗的个体中，识别肿瘤抗原的 IgM 数量减少。讨论了这些抗体是否是适应性反应的高亲和力产物的可能性。还评估了 IgG 的识别，但在对照和用 4T1 细胞处理的个体中均未发现 IgG 的识别。
