1 Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa.
2 Next Generation Sequencing Unit, Department of Medical Virology, Faculty of Health Sciences, University of the Free State, Bloemfontein, South Africa.
J Gen Virol. 2019 Jun;100(6):932-937. doi: 10.1099/jgv.0.001270. Epub 2019 May 29.
A high prevalence of G12 rotavirus strains has previously been reported in southern Mozambique. In this study, the full genomes of five Mozambican G12 strains were determined directly from stool using an Illumina Miseq platform. One sample (0060) contained an intergenogroup co-infection of a G12P[8] Wa-like strain and a GXP[14] DS-1-like strain. The sequences of seven genome segments, detected for the GXP[14] strain, clustered with a diverse group of mostly animal strains, suggesting co-infection with a strain of possible animal origin. The stool samples contained G12P[6] rotavirus strains with Wa-like backbones. Phylogenetic analyses of the VP4 and VP7 encoding segments of the G12P[6] strains suggested that they were reassortants containing backbones that are similar to that of the G12P[8] strain. The study confirms previous observations of interspecies transmission and emphasizes the importance of whole-genome sequencing in order to evaluate rotavirus co-infections and reassortants.
此前在莫桑比克南部已报道 G12 轮状病毒株的高流行率。在本研究中，使用 Illumina Miseq 平台直接从粪便中确定了五株莫桑比克 G12 株的全长基因组。一个样本（0060）含有 G12P[8] Wa 样株和 GXP[14] DS-1 样株的种间共同感染。对于 GXP[14] 株检测到的七个基因组片段的序列与大多数动物株的多样化群聚类，表明与可能来自动物的株共同感染。粪便样本中含有 Wa 样骨架的 G12P[6] 轮状病毒株。G12P[6] 株的 VP4 和 VP7 编码片段的系统发育分析表明，它们是包含与 G12P[8] 株相似骨架的重组体。该研究证实了种间传播的先前观察结果，并强调了进行全基因组测序以评估轮状病毒共同感染和重组体的重要性。
