Luetkens Julian A, Klein Sabine, Träber Frank, Block Wolfgang, Schmeel Frederic C, Sprinkart Alois M, Kuetting Daniel L R, Uschner Frank E, Schierwagen Robert, Thomas Daniel, Trebicka Jonel, Kukuk Guido M
Department of Radiology, University Hospital Bonn, Venusberg-Campus 1, 53127, Bonn, Germany.
Department of Internal Medicine I, University Hospital Bonn, Venusberg-Campus 1, 53127, Bonn, Germany.
Eur Radiol Exp. 2019 May 29;3(1):22. doi: 10.1186/s41747-019-0100-y.
To determine the utility of single-contrast-bolus hepatic extracellular volume (ECV) fraction measurement at different time points to detect and quantify hepatic fibrosis.
Different grades of liver fibrosis were induced in 23 male Sprague-Dawley rats by carbon-tetrachloride (CCl) intoxication. In ten control rats, no fibrosis was induced. Native T1 values and ECV fraction were assessed by using quantitative magnetic resonance imaging (MRI) mapping; only one contrast bolus was applied (gadobutrol 0.1 mmol/kg). ECV values were determined 5, 15, and 25 min after injection. Hepatic fibrosis was quantified histologically by Sirius red staining.
For the 8-week-CCl group, the ECV fraction values obtained 5 (23.5 ± 4.8%, mean ± standard deviation), 15 (23.6 ± 4.8%), and 25 min (23.7 ± 4.7%) after injection were constant over time (p = 0.998); constant data 5-25 min after injection were also observed for the 16-week-CCl group and controls. Liver ECV after 15 min significantly increased with the severity of fibrosis: 18.0 ± 3.0% (controls) versus 23.6 ± 4.8% (8-week-CCl4) versus 30.5 ± 3.3% (16-week-CCl4) (p < 0.001). ECV values after 5, 15, and 25 min significantly correlated with Sirius red staining (p < 0.001 for all parameters).
Hepatic ECV obtained using a single-contrast-bolus technique can be measured 5, 15, and 25 min after injection, obtaining constant values over time, each of them being suitable to detect diffuse hepatic fibrosis. In clinical practice, post-contrast T1 relaxation times for liver ECV fraction determination might be obtained at only one time point.
确定在不同时间点测量单对比剂团注肝脏细胞外容积（ECV）分数以检测和量化肝纤维化的效用。
通过四氯化碳（CCl）中毒诱导23只雄性Sprague-Dawley大鼠产生不同程度的肝纤维化。10只对照大鼠未诱导纤维化。使用定量磁共振成像（MRI）图谱评估固有T1值和ECV分数；仅注射一次对比剂团注（钆布醇0.1 mmol/kg）。在注射后5、15和25分钟测定ECV值。通过天狼星红染色对肝纤维化进行组织学定量。
对于8周CCl组，注射后5分钟（23.5±4.8%，平均值±标准差）、15分钟（23.6±4.8%）和25分钟（23.7±4.7%）获得的ECV分数值随时间保持恒定（p = 0.998）；16周CCl组和对照组在注射后5至25分钟也观察到恒定数据。注射15分钟后肝脏ECV随纤维化严重程度显著增加：18.0±3.0%（对照组）对23.6±4.8%（8周CCl4组）对30.5±3.3%（16周CCl4组）（p < 0.001）。5、15和25分钟后的ECV值与天狼星红染色显著相关（所有参数p < 0.001）。
使用单对比剂团注技术获得的肝脏ECV可在注射后5、15和25分钟测量，随时间获得恒定值，每个值都适合检测弥漫性肝纤维化。在临床实践中，测定肝脏ECV分数的对比后T1弛豫时间可能仅在一个时间点获得。
