Department of Bioengineering, Faculty of Engineering, Ege University, 35100 Bornova, Izmir, Turkey.
Department of Genetics & Bioengineering, Faculty of Engineering, Gumushane University, 29100 Gumushane, Turkey.
Food Chem. 2019 Sep 30;293:57-65. doi: 10.1016/j.foodchem.2019.04.086. Epub 2019 Apr 25.
The bioavailability of drugs can be improved by regulating the structural properties, particularly lipoid systems, such as niosomes, can increase cellular uptake. Herein, we optimized double emulsion and niosomal formulations for encapsulating anthocyanin-rich black carrot extract. Nanoparticles obtained by selected formulation were characterized in terms of morphology, particle size, drug encapsulation efficiency, in vitro release and cytotoxicity. The optimum conditions for niosomal formulation were elicited as 30 mg of cholesterol, 150 mg of Tween 20 and feeding time of 1 min at a stirring rate of 900 rpm yielding the lowest average particle size of 130 nm. In vitro release data showed the majority of the encapsulated anthocyanins were released at the end of 10 h. A mathematical model was developed to estimate the absorption of anthocyanins released from niosomes and cytotoxicity was assessed against neuroblastoma. Overall, these findings suggest that niosomal vesicles might be suitable delivery systems for anthocyanins.
药物的生物利用度可以通过调节结构特性来提高，特别是脂质体系统，如囊泡，可以增加细胞摄取。本文优化了包封富含花色苷的黑胡萝卜提取物的复乳和囊泡制剂。通过选择的配方获得的纳米粒子在形态、粒径、药物包封效率、体外释放和细胞毒性方面进行了表征。囊泡制剂的最佳条件为胆固醇 30mg、吐温 20 150mg 和搅拌速度为 900rpm 时的进料时间为 1min，得到的平均粒径最低为 130nm。体外释放数据表明，大多数包封的花色苷在 10 小时结束时释放。建立了一个数学模型来估计从囊泡中释放的花色苷的吸收，并评估了对神经母细胞瘤的细胞毒性。总的来说，这些发现表明囊泡可能是花色苷的合适递送系统。
