Sheng Xiaoling, Chen Huanwei, Wang Jianmei, Zheng Yongli, Li Yueling, Jin Zexin, Li Junmin
School of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou University, Taizhou 318000, China.
ACS Omega. 2021 Mar 28;6(13):9028-9038. doi: 10.1021/acsomega.1c00059. eCollection 2021 Apr 6.
Flavonoids are a class of commonly occurring natural compounds in the plant kingdom with various biological activities. This study compares the content of flavonoids in at different developmental stages to better inform the selection of the optimal picking period. Thus, we analyzed the transcriptome and metabolome of at different developmental stages. The transcriptome analysis revealed 44 genes involved in the biosynthesis of flavonoids in , with 10 differentially expressed genes across the four different developmental stages. The metabolites were separated and identified by a combination of chromatography and mass spectrometry, followed by multi-reaction monitoring mode analysis of triple quadrupole mass spectrometry for complete metabolite quantification. In the flavonoid synthesis pathway, a total of 137 differential flavonoids were detected. The joint transcriptome and metabolome analysis showed that the expression trends in differential metabolites and genes were significantly related. Four MYB transcription factors and two bHLH transcription factors that are closely related to flavonoid biosynthesis were identified. The regulation network of flavonoid biosynthesis in was thus established, providing guidance for follow-up research.
黄酮类化合物是植物界中一类常见的天然化合物，具有多种生物活性。本研究比较了[植物名称]在不同发育阶段的黄酮类化合物含量，以便更好地为最佳采摘期的选择提供依据。因此，我们分析了[植物名称]在不同发育阶段的转录组和代谢组。转录组分析揭示了[植物名称]中44个参与黄酮类化合物生物合成的基因，其中有10个差异表达基因分布在四个不同的发育阶段。通过色谱和质谱联用对代谢物进行分离和鉴定，随后采用三重四极杆质谱的多反应监测模式分析对代谢物进行完整定量。在黄酮类化合物合成途径中，共检测到137种差异黄酮类化合物。转录组和代谢组联合分析表明，差异代谢物和基因的表达趋势显著相关。鉴定出了4个与黄酮类化合物生物合成密切相关的MYB转录因子和2个bHLH转录因子。由此建立了[植物名称]中黄酮类化合物生物合成的调控网络，为后续研究提供了指导。
