Mertz P M, Patti J M, Marcin J J, Marshall D A
Department of Dermatology and Cutaneous Surgery, University of Miami School of Medicine, Florida 33101.
J Clin Microbiol. 1987 Sep;25(9):1601-4. doi: 10.1128/jcm.25.9.1601-1604.1987.
The adherence of bacterial pathogens to wounded skin is probably the first step in wound infection. This report describes the development of a bioassay to simulate the adherence of bacteria to wounds. The adherence of bacteria was examined by exposing wounds to known quantities of pathogens, washing the wounds with distilled water, and quantitating the number of adherent bacteria per cm2 of tissue. Our studies focused on the effects of naturally occurring mediators of bacterial adherence, such as wound fluid, serum, and fibronectin. Bacterial adherence was shown to be challenge dependent. Addition of wound fluid, serum, and heat-inactivated serum was shown to reduce the adherence of Pseudomonas aeruginosa to the wound surface compared with that of a saline control. Additional fibronectin treatment of the wound had no effect on the adherence of Staphylococcus aureus. The ability to identify what affects the binding of bacteria to wounded skin can lead to a better understanding of wound infection.
细菌性病原体对受伤皮肤的黏附可能是伤口感染的第一步。本报告描述了一种生物测定法的开发，用于模拟细菌对伤口的黏附。通过将伤口暴露于已知数量的病原体、用蒸馏水冲洗伤口以及定量每平方厘米组织上黏附细菌的数量来检测细菌的黏附情况。我们的研究集中在细菌黏附的天然介质，如伤口渗出液、血清和纤连蛋白的作用上。结果表明细菌黏附具有挑战性依赖性。与生理盐水对照相比，添加伤口渗出液、血清和热灭活血清可降低铜绿假单胞菌对伤口表面的黏附。额外用纤连蛋白处理伤口对金黄色葡萄球菌的黏附没有影响。识别影响细菌与受伤皮肤结合的因素的能力有助于更好地理解伤口感染。
