Mitsuma T, Sun D H, Nogimori T, Chaya M, Ohtake K, Hirooka Y
Fourth Department of Internal Medicine, Aichi Medical University, Japan.
Peptides. 1987 May-Jun;8(3):473-5. doi: 10.1016/0196-9781(87)90012-x.
Effects of histamine and related compounds on the release of immunoreactive thyrotropin-releasing hormone (ir-TRH) from the rat stomach in vitro were studied. The rat stomach was incubated in medium 199 with 1.0 mg/ml of bacitracin and 100 micrograms/ml of ascorbic acid (pH 7.4) for 20 min. The amount of TRH release into the medium was measured by radioimmunoassay. The ir-TRH release from the rat stomach was enhanced significantly in a dose-related manner with the addition of histamine and inhibited with the addition of famotidine, but not with mepyramine. The stimulatory effect of histamine on ir-TRH release from the stomach was partially blocked with the addition of famotidine, but not with mepyramine. The elution profile of acid-methanol-extracted rat stomach on Sephadex G-10 was identical to that of synthetic TRH. These findings suggest that histamine stimulated ir-TRH release from the rat stomach in vitro, and that histamine's effects may be mediated via a H2-receptor.
研究了组胺及相关化合物对大鼠胃组织在体外释放免疫反应性促甲状腺激素释放激素（ir-TRH）的影响。将大鼠胃组织置于含有1.0 mg/ml杆菌肽和100 μg/ml抗坏血酸（pH 7.4）的199培养基中孵育20分钟。通过放射免疫分析法测定释放到培养基中的TRH量。添加组胺后，大鼠胃组织中ir-TRH的释放呈剂量依赖性显著增强，添加法莫替丁可抑制其释放，而美吡拉敏则无此作用。组胺对胃组织ir-TRH释放的刺激作用，在添加法莫替丁后部分受到阻断，而美吡拉敏则无此作用。用葡聚糖凝胶G-10对酸-甲醇提取的大鼠胃组织进行洗脱，其洗脱图谱与合成TRH相同。这些结果表明，组胺在体外刺激大鼠胃组织释放ir-TRH，且组胺的作用可能通过H2受体介导。
