内皮素-1对大鼠胃中生长抑素和促甲状腺激素释放激素释放的体外作用。

In vitro effects of endothelin-1 on somatostatin and thyrotropin-releasing hormone release from the rat stomach.

作者信息

Mitsuma T, Kaneko H, Hirooka Y, Mori S, Mori Y, Adachi K, Yamamoto S, Hayakawa T, Yamaguchi C, Uruma M, Rhue N, Ping J, Nogimori T

机构信息

The Fourth Department of Internal Medicine, Aichi Medical University, Aichi, Japan.

出版信息

Life Sci. 1998;62(11):1007-12. doi: 10.1016/s0024-3205(98)00021-6.

DOI:10.1016/s0024-3205(98)00021-6

PMID:9515558

Abstract

The effects of endothelin (ET) 1 on the release of somatostatin (SS) and thyrotropin-releasing hormone (TRH) from the rat stomach were studied in vitro. The rat stomach was incubated in medium 199 with 1.0 mg/ml of bacitracin (pH 7.4) for 20 min. The amounts of SS and TRH released into the medium were measured by individual radioimmunoassays. With the addition of ET-1, the release of SS from the rat stomach was inhibited significantly in a dose-related manner, whereas TRH released from the stomach was enhanced significantly. These effects of ET-1 on SS or TRH release were blocked by BQ-485, a blocker of ETA receptor. These findings suggest that ET-1 inhibits SS and stimulates TRH release from the rat stomach in vitro, and that these effects are mediate via ETA receptor.

摘要

在体外研究了内皮素（ET）-1对大鼠胃中生长抑素（SS）和促甲状腺激素释放激素（TRH）释放的影响。将大鼠胃在含有1.0mg/ml杆菌肽（pH 7.4）的199培养基中孵育20分钟。通过单独的放射免疫测定法测量释放到培养基中的SS和TRH的量。加入ET-1后，大鼠胃中SS的释放以剂量相关的方式被显著抑制，而胃中释放的TRH则显著增加。ET-1对SS或TRH释放的这些作用被ETA受体阻滞剂BQ-485阻断。这些发现表明，ET-1在体外抑制大鼠胃中SS的释放并刺激TRH的释放，并且这些作用是通过ETA受体介导的。