Mitsuma T, Kaneko H, Hirooka Y, Mori S, Mori Y, Adachi K, Yamamoto S, Hayakawa T, Yamaguchi C, Uruma M, Rhue N, Ping J, Nogimori T
The Fourth Department of Internal Medicine, Aichi Medical University, Aichi, Japan.
Life Sci. 1998;62(11):1007-12. doi: 10.1016/s0024-3205(98)00021-6.
The effects of endothelin (ET) 1 on the release of somatostatin (SS) and thyrotropin-releasing hormone (TRH) from the rat stomach were studied in vitro. The rat stomach was incubated in medium 199 with 1.0 mg/ml of bacitracin (pH 7.4) for 20 min. The amounts of SS and TRH released into the medium were measured by individual radioimmunoassays. With the addition of ET-1, the release of SS from the rat stomach was inhibited significantly in a dose-related manner, whereas TRH released from the stomach was enhanced significantly. These effects of ET-1 on SS or TRH release were blocked by BQ-485, a blocker of ETA receptor. These findings suggest that ET-1 inhibits SS and stimulates TRH release from the rat stomach in vitro, and that these effects are mediate via ETA receptor.
在体外研究了内皮素（ET）-1对大鼠胃中生长抑素（SS）和促甲状腺激素释放激素（TRH）释放的影响。将大鼠胃在含有1.0mg/ml杆菌肽（pH 7.4）的199培养基中孵育20分钟。通过单独的放射免疫测定法测量释放到培养基中的SS和TRH的量。加入ET-1后，大鼠胃中SS的释放以剂量相关的方式被显著抑制，而胃中释放的TRH则显著增加。ET-1对SS或TRH释放的这些作用被ETA受体阻滞剂BQ-485阻断。这些发现表明，ET-1在体外抑制大鼠胃中SS的释放并刺激TRH的释放，并且这些作用是通过ETA受体介导的。
