全细胞 cAMP 和 PKA 活性只是表象，纳米域信号传递才是关键。
Whole-Cell cAMP and PKA Activity are Epiphenomena, Nanodomain Signaling Matters.
机构信息
Department of Pharmacology, University of California , Davis, California.
Department of Physiology, Anatomy and Genetics, University of Oxford , Oxford , United Kingdom.
出版信息
Physiology (Bethesda). 2019 Jul 1;34(4):240-249. doi: 10.1152/physiol.00002.2019.
Abstract
Novel targeted fluorescent biosensors provide key insights into very local nanodomains of cAMP and PKA activity, and how they respond differently to β-adrenergic activation in cardiac myocytes. This unique spatiotemporal detail in living cells is not available with biochemical measurements of total cellular cAMP and PKA, and provides unique physiological insights.
摘要
新型靶向荧光生物传感器为研究 cAMP 和 PKA 活性的非常局部纳米域提供了重要的见解，以及它们在心肌细胞中对β-肾上腺素能激活的反应如何不同。与总细胞 cAMP 和 PKA 的生化测量相比，活细胞中这种独特的时空细节不可用，并且提供了独特的生理见解。
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