Shimizu T, Homma J Y, Abe C, Tanamoto T, Aoyama Y, Yanagawa R, Fujimoto Y, Noda H, Takashima I, Honda E, Minamide S
Am J Vet Res. 1976 Dec;37(12):1441-4.
The protein portion of endotoxin (OEP), or common antigen of Pseudomonas aeruginosa prepared from strain N10 of P aeruginosa, was examined as a possible vaccine against this organism in mink. Mink were given OEP vaccine and challenge exposed by instillation with the inoculum into the nostril. They survived after this exposure with 4 median lethal (LD50) doses of strain NC-5 (protease negative, elastase negative) or with 160 LD50 doses of strain 5 (protease positive, elastase positive). Strain NC-5 belonged to TYPE 5 AND strain 5 belonged to type 8 (according to the serotyping method of Homma). Strain N10, the source of OEP, belonged to serotype 5. Thus, the mink vaccinated with OEP vaccine were able to stand challenge exposure to both homologous and heterologous serotype strains. More mink given OEP vaccine survived the challenge exposure by intranasal spraying with virulent strain 5 than did those in the nonvaccinated control group, with a significant difference. When the surviving mink of the vaccinated group were rechallenge exposed with 300 or 3,000 LD50 doses of the same strain a month later, most of them survived.
对铜绿假单胞菌N10菌株制备的内毒素蛋白部分（OEP）或铜绿假单胞菌共同抗原作为水貂抗该菌的一种可能疫苗进行了研究。给水貂接种OEP疫苗，并通过将接种物滴入鼻孔进行攻击暴露。在暴露于4个NC - 5菌株（蛋白酶阴性、弹性蛋白酶阴性）的半数致死剂量（LD50）或160个5菌株（蛋白酶阳性、弹性蛋白酶阳性）的LD50剂量后，它们存活了下来。NC - 5菌株属于5型，5菌株属于8型（根据本间的血清分型方法）。OEP的来源菌株N10属于血清型5。因此，接种OEP疫苗的水貂能够经受住同源和异源血清型菌株的攻击暴露。与未接种疫苗的对照组相比，更多接种OEP疫苗的水貂在通过鼻内喷雾感染强毒株5的攻击暴露中存活下来，差异显著。当接种组存活的水貂在一个月后再次接受300或3,000个LD50剂量的同一菌株攻击暴露时，它们中的大多数存活了下来。
