Department of Clinical Sciences, College of Veterinary Medicine, Colorado State University, Fort Collins, Colorado.
Department of Pathobiology and Population Sciences, Royal Veterinary College, Hatfield, United Kingdom.
J Vet Intern Med. 2019 Jul;33(4):1669-1676. doi: 10.1111/jvim.15545. Epub 2019 Jun 6.
Lymphatic endothelial cell (LEC) immunohistochemical markers have identified intestinal lymphatic vasculature abnormalities in humans with inflammatory bowel disease, but have not been used to evaluate intestinal lymphatic vasculature in a group of dogs with chronic inflammatory enteropathy (CIE).
To utilize LEC markers to identify and measure intestinal lymphatic vasculature in endoscopic biopsy samples of CIE dogs. To evaluate whether measured lymphatic vasculature variables correlate with serum albumin concentrations.
Twenty-four dogs with CIE; n = 13, serum albumin concentration <2.5 g/dL (CIE-protein-losing enteropathy [PLE]), n = 11, serum albumin concentration ≥2.5 g/dL (CIE-N).
Prospective study. Lymphatic endothelial cell immunolabeling with Prox-1 and LYVE-1 performed on endoscopic biopsy samples from 24 dogs with CIE. Duodenal and ileal villous lacteal width (VLW) and proprial mucosal lacteal width (MLW) were determined for each case and analyzed for correlation with serum albumin concentration. Lacteal dilatation scores using routine H&E histopathology were assessed for correlation with immunohistochemistry (IHC)-calculated VLW and MLW.
Lower serum albumin concentrations were correlated with increased VLW (rho = -.4644; P = .02) and MLW (rho = -.6514; P < .001) in the ileum. Lymphatic endothelial cell IHC identified presumptive proprial mucosal lymphangiectasia in some dogs that was not recognized with routine H&E staining. Lacteal dilatation scores were correlated with VLW in duodenum (rho = .4634; P = .02) and ileum (rho = .5292; P = .008), but did not correlate with MLW.
Lymphatic endothelial cell immunolabeling identified presumptive proprial mucosal lymphangiectasia in CIE dogs, particularly in the ileum of hypoalbuminemic dogs. Routine evaluation of villous lacteals likely underestimates abnormalities of the lymphatic vasculature in dogs with CIE.
淋巴内皮细胞（LEC）免疫组织化学标志物已在患有炎症性肠病的人类中鉴定出肠道淋巴管异常，但尚未用于评估患有慢性炎症性肠病（CIE）的一组犬的肠道淋巴管。
利用 LEC 标志物识别和测量 CIE 犬内镜活检样本中的肠道淋巴管。评估测量的淋巴管变量是否与血清白蛋白浓度相关。
24 只患有 CIE 的犬；n = 13，血清白蛋白浓度<2.5 g/dL（CIE-蛋白丢失性肠病 [PLE]），n = 11，血清白蛋白浓度≥2.5 g/dL（CIE-N）。
前瞻性研究。对 24 只患有 CIE 的犬的内镜活检样本进行 Prox-1 和 LYVE-1 的淋巴内皮细胞免疫标记。确定每个病例的十二指肠和回肠绒毛乳糜管宽度（VLW）和固有黏膜乳糜管宽度（MLW），并分析其与血清白蛋白浓度的相关性。使用常规 H&E 组织病理学评估乳糜管扩张评分与免疫组织化学（IHC）计算的 VLW 和 MLW 的相关性。
血清白蛋白浓度较低与回肠 VLW（rho = -.4644；P =.02）和 MLW（rho = -.6514；P < .001）增加相关。免疫组织化学鉴定了一些犬的推定固有黏膜淋巴管扩张，常规 H&E 染色无法识别。乳糜管扩张评分与十二指肠 VLW（rho =.4634；P =.02）和回肠 VLW（rho =.5292；P =.008）相关，但与 MLW 不相关。
淋巴内皮细胞免疫标记在 CIE 犬中鉴定出推定固有黏膜淋巴管扩张，特别是在低白蛋白血症犬的回肠中。常规评估绒毛乳糜管可能低估了 CIE 犬淋巴管异常。
