Ma Kai, Li Qing-Song, Yin Cheng-Juan, Zhang Zhen-Yong
Department of Ophthalmology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China.
Int J Ophthalmol. 2021 Sep 18;14(9):1345-1349. doi: 10.18240/ijo.2021.09.08. eCollection 2021.
To investigate the effect of latanoprost eye drops on the conjunctival lymphatics.
Twenty-four healthy New Zealand White rabbits weighing 1.5 to 2.0 kg were randomly divided into three groups: latanoprost group (=8) administered with latanoprost eye drops once a day for 2mo, carteolol group (=8) administered with carteolol eye drops once a day for 2mo, and control group (=8) without any treatment. The conjunctival tissues in the three groups were extracted to investigate the expression levels of 5'-nucleotidase (5'-Nase) by Western blot, reverse transcription-polymerase chain reaction (RT-PCR), and immunofluorescence staining, respectively.
The protein expression level of 5'-Nase was significantly higher in latanoprost group than carteolol group (=231.175, <0.001) and control group (<0.001), while there was no significant difference between the carteolol group and the control group (>0.05). The mRNA expression level of 5'-Nase in the latanoprost group was also significantly higher than carteolol group (=71.169 <0.005) and control group (<0.005). The conjunctival lymphatics were positive immunofluorescence stained with the 5'-Nase antibodies in the latanoprost group and not stained in the control group.
Latanoprost eye drops can induce conjunctival lymphangiogenesis which may be concerned in clinical implications.
研究拉坦前列素滴眼液对结膜淋巴管的影响。
将24只体重1.5至2.0千克的健康新西兰白兔随机分为三组：拉坦前列素组（=8），每天滴注拉坦前列素滴眼液1次，共2个月；卡替洛尔组（=8），每天滴注卡替洛尔滴眼液1次，共2个月；对照组（=8），不进行任何治疗。分别通过蛋白质免疫印迹法、逆转录-聚合酶链反应（RT-PCR）和免疫荧光染色法提取三组的结膜组织，以研究5'-核苷酸酶（5'-Nase）的表达水平。
拉坦前列素组5'-Nase的蛋白表达水平显著高于卡替洛尔组（=231.175，<0.001）和对照组（<0.001），而卡替洛尔组和对照组之间无显著差异（>0.05）。拉坦前列素组5'-Nase的mRNA表达水平也显著高于卡替洛尔组（=71.169 <0.005）和对照组（<0.005）。拉坦前列素组结膜淋巴管被5'-Nase抗体免疫荧光染色呈阳性，而对照组未染色。
拉坦前列素滴眼液可诱导结膜淋巴管生成，这可能具有临床意义。
