从23种血培养培养基中分离出流感嗜血杆菌。
Recovery of Haemophilus influenzae from twenty-three blood culture media.
作者信息
Schell R F, Le Frock J L, Babu J P, Robinson D B
出版信息
J Clin Microbiol. 1979 Jan;9(1):84-7. doi: 10.1128/jcm.9.1.84-87.1979.
Abstract
Haemophilus influenzae is an important agent of bacteremia and has fastidious growth requirements. The purpose of this investigation was to determine the ability of commercial blood culture media to support the growth of this fastidious microorganism. Twenty-three types of blood culture media were inoculated with individual suspensions of eight strains of H. influenzae in the presence or absence of an erythrocyte-serum mixture. The rates of recovery of the H. influenzae strains from the various types of blood culture media were compared. The results demonstrated that the type of medium, the manufacturer, the erythrocyte-serum mixture, and the strain of H. influenzae influenced the recovery rates of H. influenzae. Optimal recovery of the strains of H. influenzae was obtained from brain heart infustion blood culture medium (GIBCO). Trypic soy broth (GIBCO) and supplemental peptone of Becton, Dickinson and Co. also were found to be superior to the remaining types of media tested for the recovery of H. influenzae.
摘要
流感嗜血杆菌是菌血症的重要病原体，且生长要求苛刻。本研究的目的是确定市售血培养培养基支持这种苛养微生物生长的能力。在有或没有红细胞 - 血清混合物存在的情况下，用8株流感嗜血杆菌的单个悬液接种23种血培养培养基。比较了从各种类型血培养培养基中回收流感嗜血杆菌菌株的比率。结果表明，培养基类型、制造商、红细胞 - 血清混合物以及流感嗜血杆菌菌株都会影响流感嗜血杆菌的回收率。从脑心浸液血培养培养基（GIBCO）中获得了流感嗜血杆菌菌株的最佳回收率。胰蛋白胨大豆肉汤（GIBCO）和Becton, Dickinson and Co.公司的补充蛋白胨在回收流感嗜血杆菌方面也优于其余测试的培养基类型。
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