Rubin L G, Baranowski J
J Clin Microbiol. 1985 Nov;22(5):815-8. doi: 10.1128/jcm.22.5.815-818.1985.
The isolator 1.5 Microbial Tube (E. I. du Pont de Nemours & Co., Inc., Wilmington, Del.) is a new blood culture system for detection of bacteremia in children which involves lysis of blood cells and direct plating of blood lysate on agar media. Haemophilus influenzae type b is a major pediatric pathogen and may be associated with low-density bacteremia (less than or equal to 50 CFU per ml), yet the clinical data on the efficacy of this system for recovery of H. influenzae b are limited. We used a rat model of H. influenzae b bacteremia and fresh, whole human blood inoculated in vitro with H. influenzae b to compare the 1.5 Microbial Tube and conventional Trypticase soy broth (BBL Microbiology Systems, Cockeysville, Md.) for the detection of low-density H. influenzae b bacteremia. There was a significantly lower recovery of H. influenzae b from blood of bacteremic rats and from in vitro-inoculated human blood by using the 1.5 Microbial Tube than by using Trypticase soy broth. Studies on the mechanism of this lower recovery suggested that the contents of the 1.5 Microbial Tube are toxic to H. influenzae b in the presence of human blood. Additional clinical data are necessary before the 1.5 Microbial Tube can be recommended for detection of low-density H. influenzae b bacteremia.
隔离器1.5微生物培养管（E. I. 杜邦公司，特拉华州威尔明顿市）是一种用于检测儿童菌血症的新型血培养系统，该系统涉及血细胞裂解并将血液裂解物直接接种于琼脂培养基上。b型流感嗜血杆菌是一种主要的儿科病原体，可能与低密度菌血症（每毫升小于或等于50 CFU）相关，但关于该系统对b型流感嗜血杆菌分离效果的临床数据有限。我们使用b型流感嗜血杆菌菌血症大鼠模型以及在体外接种了b型流感嗜血杆菌的新鲜人全血，比较了1.5微生物培养管与传统的胰蛋白酶大豆肉汤（BBL微生物系统公司，马里兰州科基斯维尔市）对低密度b型流感嗜血杆菌菌血症的检测能力。使用1.5微生物培养管从菌血症大鼠血液和体外接种的人血中分离出的b型流感嗜血杆菌数量显著低于使用胰蛋白酶大豆肉汤分离出的数量。对这种较低分离率机制的研究表明，在存在人血的情况下，1.5微生物培养管中的成分对b型流感嗜血杆菌具有毒性。在推荐使用1.5微生物培养管检测低密度b型流感嗜血杆菌菌血症之前，还需要更多的临床数据。
