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伊拉克埃尔比勒市生乳中分离株的分离、生物分型及药敏试验

Isolation, biotyping and antimicrobial susceptibility of isolates from raw milk in Erbil city, Iraq.

作者信息

Almashhadany Dhary Alewy

机构信息

Knowledge University Research Center, Erbil, Kurdistan Region, Iraq.

出版信息

Ital J Food Saf. 2021 Mar 11;10(1):8589. doi: 10.4081/ijfs.2021.8589.

Abstract

This study aimed to determine the prevalence and antimicrobial sensitivity of species in raw milk sold at retail vending in Erbil city. Three hundred and fifty (350) samples were aseptically collected from retail raw milk shops between January and June 2019. For isolation of spp., samples were cultured on selective media and tested for biotype and antimicrobials susceptibility by disk diffusion assay. The overall prevalence of spp. was 12.6%. was significantly prevalent (65.9%) among other species. Antimicrobial susceptibility testing showed complete sensitivity to tetracycline, rifampicin, and neomycin. On the other hand, total resistance to ampicillin and trimethoprim was observed. Strikingly, as low as 56.8% and 72.7% of isolates are still sensitive to the drugs of choice in campylobacteriosis treatment; ciprofloxacin and erythromycin respectively. This resistance pattern of found in this study is critically alarming owing to the insusceptibility to the aforementioned antibiotics commonly used as the drugs of choice for campylobacteriosis treatment. Increase in prevalence in raw milk was associated with warm season. These levels prevalence and resistance worth further investigations and effective countermeasures owing to potential public health hazards.

摘要

本研究旨在确定埃尔比勒市零售自动售货处销售的生牛奶中各物种的流行情况及抗菌敏感性。2019年1月至6月期间,从零售生牛奶商店无菌采集了350份样本。为分离各物种,将样本接种于选择性培养基上,并通过纸片扩散法检测生物型和抗菌药物敏感性。各物种的总体流行率为12.6%。在其他物种中,某物种显著流行(65.9%)。抗菌药物敏感性测试显示对四环素、利福平及新霉素完全敏感。另一方面,观察到对氨苄西林和甲氧苄啶的完全耐药。令人惊讶的是,分别仅有56.8%和72.7%的分离株对弯曲菌病治疗的首选药物环丙沙星和红霉素仍敏感。本研究中发现的该物种的这种耐药模式令人极为担忧,因为其对上述常用于弯曲菌病治疗的首选抗生素不敏感。生牛奶中该物种流行率的增加与温暖季节有关。鉴于潜在的公共卫生危害,这些流行率和耐药水平值得进一步调查并采取有效对策。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e33f/7970396/dafff3a255da/ijfs-10-1-8589-g001.jpg

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