Ben Amor Safia, Mekious Scherazad, Allal Benfekih Leila, Abdellattif Magda H, Boussebaa Walid, Almalki Faisal A, Ben Hadda Taibi, Kawsar Sarkar M A
Laboratory for Research on Medicinal and Aromatic Plants, Faculty of Nature Sciences and Life, Saad Dahlab University, Blida 1, Route de Soumâa, Blida 09000, Algeria.
Faculty of Nature Sciences and Life, Ziane Achour University, Djelfa 17000, Algeria.
Life (Basel). 2022 Jun 21;12(7):927. doi: 10.3390/life12070927.
Despite the challenging conditions in the pre-Saharan areas of Algeria, such as weak plant cover and a harsh climate, beekeeping is being developed and spread. In the present work, honey samples collected from ten locations in the El Oued region were examined during the spring of 2021. A melissopalynological analysis was carried out, followed by a floristic investigation. The 10 honey samples were also investigated for their physicochemical properties and antioxidant and antibacterial activity against five strains: , , , and The floristic analysis found 65 species belonging to 33 botanical families, with a dominance of the Asteraceae family accounting for 18.461% of the total. The melissopalynological study revealed only one monofloral honey (), whereas the nine others were multi-floral. The honey's color changed from light to dark amber, and most tested honey was of high quality, fulfilling international criteria. The total phenol and flavonoid contents varied considerably amongst the various honey samples. Furthermore, LC-MS-MS phenolic profile analysis identified the presence of 20 chemicals, of which only three phenols were found in all honey types. Antioxidant capacity analyzed with FRAP test and antiradical activities against DPPH differed from one honey sample to another. Moreover, a significant correlation was recorded between the antioxidant activity, honey's color, polyphenol, and flavonoid contents. The strain was the most sensitive regarding honey antibacterial activity, while and strains were only moderately sensitive.
尽管阿尔及利亚撒哈拉沙漠前地区条件艰苦，如植被覆盖薄弱和气候恶劣，但养蜂业仍在发展和推广。在本研究中，于2021年春季对从瓦迪地区十个地点采集的蜂蜜样本进行了检测。进行了蜂蜜花粉分析，随后进行了植物区系调查。还对这10个蜂蜜样本的理化性质以及对五种菌株（ 、 、 、 和 ）的抗氧化和抗菌活性进行了研究。植物区系分析发现65种植物属于33个植物科，其中菊科占主导，占总数的18.461%。蜂蜜花粉学研究仅发现一种单花蜂蜜（ ），而其他九种为多花蜂蜜。蜂蜜颜色从浅琥珀色变为深琥珀色，大多数测试蜂蜜质量较高，符合国际标准。不同蜂蜜样本中总酚和黄酮含量差异很大。此外，液相色谱 - 质谱 - 质谱酚类谱分析确定了20种化学物质的存在，其中所有蜂蜜类型中仅发现三种酚类。用FRAP试验分析的抗氧化能力以及对DPPH的抗自由基活性因蜂蜜样本而异。此外，抗氧化活性、蜂蜜颜色、多酚和黄酮含量之间存在显著相关性。在蜂蜜抗菌活性方面， 菌株最敏感，而 和 菌株仅中度敏感。
