Department of Chemistry, College of Science, King Khalid University, Abha, Saudi Arabia.
Sci Rep. 2024 Apr 15;14(1):8720. doi: 10.1038/s41598-024-59359-y.
This article examined the effect of geographical (different climate conditions) and floral origins on some quality parameters of honey including the activity of diastase enzyme. Moreover, some non-quality parameters were investigated such as the pH, fructose, glucose, ratio of fructose/glucose and invertase. The honey samples were collected from Asir (cold climate) and Jazan (hot climate) regions at the southwestern part of Saudi Arabia. The geographical origin significantly affected the mean value moisture of the Acacia honey (p-value = 0.02), conductivity of the polyfloral honey (p-value = 0.03), sucrose of the Acacia honey (p-value = 0.02), diastase activity of the Acacia (p-value = 0.001), Ziziphus (p-value = 0.046) and polyfloral honey (p-value ≤ 0.001), fructose of the Acacia honey (p-value = 0.01), glucose of the Ziziphus honey (p-value = 0.03), fructose/ glucose ratio of the Ziziphus honey (p-value = 0.035), and invertase activity of the polyfloral honey (p-value ≤ 0.001). Regarding the effect of the floral origin of the honey from Asir region, the sucrose percentage of the Acacia honey was significantly more than that of the polyfloral honey (p- value = 0.003), the diastase activity of the Acacia honey was significantly more than its activity in the Ziziphus honey (p- value = 0.044), glucose percentage of the Ziziphus honey was significantly more the glucose percentage of the Acacia honey (p-value = 0.009) and the fructose/ glucose ratio of the Ziziphus honey was significantly more than that of the Acacia and polyforal honeys (p-value = 0.011 and p-value = 0.045, respectively). Concerning the significant effects of the floral origin on the quality parameters of the honey samples from Jazan region, the moisture of the Ziziphus honey was significantly increased when compared to the moisture of the Acacia honey (p-value = 0.038), the acidity of the polfloral honey was significantly more than the acidity of the Acacia honey (p-value = 0.049), the sum of fructose and glucose of the polyfloral honey was significantly increased compared to that of the Acacia honey (p-value = 0.015), the pH of the Ziziphus hiney was significantly more than the pH of the polyfloral honey (0.011) and the fructose of the polfloral honey was significantly more than that of the Acacia honey (p-value = 0.031). The effect of the geographical origin of the honey samples on their quality parameters depends on their floral origin and the effect of their floral origin differs according to their geographical origin. This article suggests considering collectively the geographical and floral origins effect when developing honey standards. However, the Codex standards for honey started considering this issue when it changed the standard concentration of HMF in honey from not more than 80-40 mg/Kg for honeys from cold climate and 80 mg/Kg for honeys from hot climates.
本文研究了地理(不同气候条件)和花卉来源对蜂蜜一些质量参数的影响,包括淀粉酶酶的活性。此外,还研究了一些非质量参数,如 pH 值、果糖、葡萄糖、果糖/葡萄糖的比值和转化酶。蜂蜜样本取自沙特阿拉伯西南部的阿西尔(寒冷气候)和吉赞(炎热气候)地区。地理来源显著影响了阿拉伯胶蜂蜜的平均水分(p 值=0.02)、多花蜂蜜的电导率(p 值=0.03)、阿拉伯胶蜂蜜的蔗糖(p 值=0.02)、阿拉伯胶(p 值=0.001)、枣(p 值=0.046)和多花蜂蜜的淀粉酶活性(p 值≤0.001)、阿拉伯胶蜂蜜的果糖(p 值=0.01)、枣蜂蜜的葡萄糖(p 值=0.03)、枣蜂蜜的果糖/葡萄糖比值(p 值=0.035),以及多花蜂蜜的转化酶活性(p 值≤0.001)。至于阿西尔地区蜂蜜的花卉来源对其质量参数的影响,阿拉伯胶蜂蜜的蔗糖含量明显高于多花蜂蜜(p 值=0.003),阿拉伯胶蜂蜜的淀粉酶活性明显高于枣蜂蜜(p 值=0.044),枣蜂蜜的葡萄糖含量明显高于阿拉伯胶蜂蜜(p 值=0.009),枣蜂蜜的果糖/葡萄糖比值明显高于阿拉伯胶和多花蜂蜜(p 值=0.011 和 p 值=0.045)。至于吉赞地区蜂蜜样本质量参数的花卉来源的显著影响,枣蜂蜜的水分明显高于阿拉伯胶蜂蜜(p 值=0.038),多花蜂蜜的酸度明显高于阿拉伯胶蜂蜜(p 值=0.049),多花蜂蜜的果糖和葡萄糖之和明显高于阿拉伯胶蜂蜜(p 值=0.015),枣蜂蜜的 pH 值明显高于多花蜂蜜(0.011),多花蜂蜜的果糖含量明显高于阿拉伯胶蜂蜜(p 值=0.031)。蜂蜜样本质量参数的地理来源的影响取决于其花卉来源,而其花卉来源的影响则取决于其地理来源。本文建议在制定蜂蜜标准时,综合考虑地理和花卉来源的影响。然而,蜂蜜法典标准在改变寒冷气候地区蜂蜜中 HMF 的标准浓度时开始考虑这个问题,从不超过 80-40mg/Kg 变为 80mg/Kg,而炎热气候地区的蜂蜜则保持不变。