通过网络分析对结直肠癌进行基因筛查。
Gene screening of colorectal cancers via network analysis.
作者信息
Mansouri Vahid, Rezaei Tavirani Mostafa, Rezaei Tavirani Sina
机构信息
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Gastroenterol Hepatol Bed Bench. 2019 Spring;12(2):149-154.
AIM
Identifying crucial genes related to colorectal cancers via protein-protein interaction (PPI) network analysis is the aim of this study.
BACKGROUND
colorectal cancer as major reason of mortality is evaluated by genetic and proteomic approaches to find suitable biomarkers. Chromosomal instability plays crucial role in CRC. Expression change of large numbers of genes is reported.
METHODS
Differentially expressed genes related to CRCs which obtained from different proteomic methods were extracted from a review article of Paula Álvarez-Chaver . The genes interacted by Cytoscape software via STRING database. The central nodes determined and were enriched for biological terms by ClueGO. Action map for central genes was illustrated by CluePedia. The critical genes in CRC were introduced.
RESULTS
Among 123 query genes, 114 one recognized by software and were included in the network. SRC, EGFR, PCNA, IL8, CTNNB1, TIMP1, CDH1, and HSPD1 were determined as central genes. After gene ontology analysis SRC, EGFR, and CDH1 were identified as critical genes related to CRC.
CONCLUSION
It seems that SRC, EGFR, and CDH1 and the related pathways are possible biomarkers for CRC.
目的
通过蛋白质-蛋白质相互作用（PPI）网络分析鉴定与结直肠癌相关的关键基因是本研究的目的。
背景
结直肠癌作为主要的死亡原因，通过基因和蛋白质组学方法进行评估以寻找合适的生物标志物。染色体不稳定性在结直肠癌中起关键作用。已有大量基因表达变化的报道。
方法
从Paula Álvarez-Chaver的一篇综述文章中提取通过不同蛋白质组学方法获得的与结直肠癌相关的差异表达基因。这些基因通过Cytoscape软件经STRING数据库进行相互作用分析。确定中心节点，并通过ClueGO对生物学术语进行富集分析。通过CluePedia展示中心基因的作用图谱。介绍了结直肠癌中的关键基因。
结果
在123个查询基因中，114个被软件识别并纳入网络。SRC、表皮生长因子受体（EGFR）、增殖细胞核抗原（PCNA）、白细胞介素8（IL8）、β-连环蛋白1（CTNNB1）、金属蛋白酶组织抑制因子1（TIMP1）、钙黏蛋白1（CDH1）和热休克蛋白D1（HSPD1）被确定为中心基因。经过基因本体分析，SRC、EGFR和CDH1被确定为与结直肠癌相关的关键基因。
结论
似乎SRC、EGFR和CDH1以及相关途径可能是结直肠癌的生物标志物。
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