胃萎缩的生化途径分析
Biochemical pathway analysis of gastric atrophy.
作者信息
Rezaei Tavirani Mostafa, Rezaei Tavirani Sina, Tajik Rostami Fatemeh
机构信息
Proteomics Research Center,Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
出版信息
Gastroenterol Hepatol Bed Bench. 2018 Spring;11(2):118-124.
AIM
Pathway analysis of gastric atrophy to find new molecular prospective of disease.
BACKGROUND
Gastric atrophy as a process which is accompanied with "loss of glans" in stomach can be considered as a risk factor of gastric cancer. Here, the correlated biochemical pathways to the disorder have been analyzed via protein-protein interaction (PPI) network analysis.
METHODS
The genes related to gastric atrophy were retrieved by STRING database and organized in a network by Cytoscape. Three significant clusters were determined by ClusterONE plug-in of Cytoscape. The elements of cluster-2 which contained all central nodes of the network were enriched by ClueGO and the biochemical pathways discussed in details.
RESULTS
The number of seven central nodes (which are included in cluster-2); INS, TP53, IL6, TNF, SRC, MYC, and IL8 were identified. The biochemical pathways related to the elements of cluster-2 were determined and clustered in nine groups. The pathways were discussed in details.
CONCLUSION
Pathway analysis indicates that the introduced central genes of the network can be considered as biomarkers of gastric atrophy.
目的
对胃萎缩进行通路分析，以寻找该疾病新的分子研究方向。
背景
胃萎缩作为一种伴有胃“腺体丢失”的过程，可被视为胃癌的一个危险因素。在此，通过蛋白质 - 蛋白质相互作用（PPI）网络分析对与该病症相关的生化通路进行了分析。
方法
通过STRING数据库检索与胃萎缩相关的基因，并利用Cytoscape将其组织成一个网络。通过Cytoscape的ClusterONE插件确定了三个显著的聚类。通过ClueGO对包含网络所有中心节点的聚类2的元素进行富集，并详细讨论了生化通路。
结果
确定了七个中心节点（包含在聚类2中）的数量；分别为胰岛素（INS）、肿瘤蛋白p53（TP53）、白细胞介素6（IL6）、肿瘤坏死因子（TNF）、原癌基因酪氨酸蛋白激酶（SRC）、原癌基因c - myc（MYC）和白细胞介素8（IL8）。确定了与聚类2元素相关的生化通路，并将其聚类为九组。对这些通路进行了详细讨论。
结论
通路分析表明，该网络中引入的中心基因可被视为胃萎缩的生物标志物。
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