Beijing Key Laboratory for Precancerous Lesion of Digestive Disease, Department of Gastroenterology, Beijing Friendship Hospital, National Clinical Research Center for Digestive Disease, Beijing Digestive Disease Center, Capital Medical University, Beijing, China.
Department of Operations and Information Management, China-Japan Friendship Hospital, Beijing, China.
Front Cell Infect Microbiol. 2019 May 29;9:178. doi: 10.3389/fcimb.2019.00178. eCollection 2019.
Irritable bowel syndrome (IBS) is a common gastrointestinal dysfunctional disease. The pathophysiology of IBS is, however, largely unknown. This study aimed to determine whether evaluation of fecal metabolite and microbiota profiles may offer an opportunity to identify a novel pathophysiological target for IBS, and to reveal possible gut microbe-metabolite associations. By using gas chromatography coupled to time-of-flight mass spectrometry (GC-TOFMS) and 16S rRNA gene sequencing, we measured fecal metabolites and microbiota of the control and water avoidance stress (WAS)-induced IBS rats. We found a significantly differential metabolite profile between the IBS and control groups; a cluster of metabolites was also found to be significantly associated with the amount of defecations. Moreover, the WAS group exhibited a decreased alpha diversity of the microbial population as compared to the control group. However, the characteristics of gut microbiota could not differentiate the IBS group from the control group. Correlation of the metabolite level with the number of microbial genera showed no significant association between the control and IBS groups. This study provides a global perspective on metabolomics and microbiota profiling in WAS-induced IBS model and a theoretical basis for research on the pathophysiology of IBS.
肠易激综合征（IBS）是一种常见的胃肠道功能紊乱疾病。然而，IBS 的病理生理学在很大程度上是未知的。本研究旨在确定评估粪便代谢物和微生物群谱是否为 IBS 提供了一个新的病理生理靶点的机会，并揭示可能的肠道微生物-代谢物关联。通过使用气相色谱-飞行时间质谱联用（GC-TOFMS）和 16S rRNA 基因测序，我们测量了对照组和水回避应激（WAS）诱导的 IBS 大鼠的粪便代谢物和微生物群。我们发现 IBS 组和对照组之间存在明显不同的代谢物谱；一组代谢物也与粪便量显著相关。此外，与对照组相比，WAS 组的微生物种群的 alpha 多样性降低。然而，肠道微生物群的特征不能将 IBS 组与对照组区分开来。代谢物水平与微生物属数量的相关性表明，对照组和 IBS 组之间没有显著关联。本研究提供了 WAS 诱导的 IBS 模型中代谢组学和微生物组学分析的全局视角，并为 IBS 病理生理学的研究提供了理论基础。
