Nicholson W L, Chambliss G H
Laboratory of Genetics, University of Wisconsin, Madison 53706.
J Bacteriol. 1987 Dec;169(12):5867-9. doi: 10.1128/jb.169.12.5867-5869.1987.
Decoyinine, an inhibitor of GMP synthetase, allows sporulation in Bacillus subtilis to initiate and proceed under otherwise catabolite-repressing conditions. The effect of decoyinine on alpha-amylase synthesis in B. subtilis, an event which exhibits regulatory features resembling sporulation initiation, was examined. Decoyinine did not overcome catabolite repression of alpha-amylase synthesis in a wild-type strain of B. subtilis but did cause premature and enhanced synthesis in a mutant strain specifically blocked in catabolite repression of alpha-amylase synthesis. Decoyinine had no effect on alpha-amylase enzymatic activity. Thus, it appears that the catabolite control mechanisms governing alpha-amylase synthesis and sporulation in B. subtilis differ in their responses to decoyinine and hence must consist at least partially of separate components.
脱氧精胍菌素是鸟苷酸合成酶的抑制剂，它能使枯草芽孢杆菌在其他情况下会受到分解代谢物阻遏的条件下启动并进行孢子形成。研究了脱氧精胍菌素对枯草芽孢杆菌中α-淀粉酶合成的影响，α-淀粉酶合成这一事件具有类似于孢子形成起始的调控特征。脱氧精胍菌素不能克服枯草芽孢杆菌野生型菌株中α-淀粉酶合成的分解代谢物阻遏，但在一个特别阻断了α-淀粉酶合成的分解代谢物阻遏的突变菌株中，它确实导致了α-淀粉酶的过早合成和增强合成。脱氧精胍菌素对α-淀粉酶的酶活性没有影响。因此，似乎在枯草芽孢杆菌中，控制α-淀粉酶合成和孢子形成的分解代谢物控制机制对脱氧精胍菌素的反应不同，因此至少部分由不同的成分组成。
