Yuan Shenglei, Wang Yundan, Zhao Fangqing, Kang Le
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, China.
Front Microbiol. 2021 Sep 28;12:749218. doi: 10.3389/fmicb.2021.749218. eCollection 2021.
The genus is attracting an increasing amount of attention because of its multiple functions and probiotic potential. In particular, the species is known to have great potential in industrial applications and exhibits numerous biological functions. However, the knowledge on this bacterium in insects is not investigated. Here, we isolated and identified as the dominant lactic acid bacteria in the gut of the migratory locust. We named this strain LM1, which is the first genome of an insect-derived strain with one complete chromosome and one complete plasmid. Among all strains, LM1 had the largest genome. Its genome was the closest to that of 1001271B_151109_G12, a strain from human feces. Our results provided accurate evolutionary relationships of known species and strains. Based on genomic analysis, the pan-genome of is in an open state. Most strains of had the unique genes, indicating that these strains can adapt to different ecological niches and organisms. However, the variation of strain-specific genes did represent significant correlations with their hosts and ecological niches. These strains were predicted to have low potential to produce secondary metabolites. Furthermore, no antibiotic resistance genes were identified. At the same time, virulence factors associated with toxin production and secretion system were not found, indicating that strains were not sufficient to perform virulence. Our study facilitated the discovery of the functions of LM1 in locust biology and their potential application to locust management.
由于其多种功能和益生菌潜力，该属正吸引着越来越多的关注。特别是，已知该物种在工业应用中具有巨大潜力，并展现出众多生物学功能。然而，关于这种细菌在昆虫中的情况尚未得到研究。在此，我们分离并鉴定出该菌为飞蝗肠道中的优势乳酸菌。我们将此菌株命名为LM1，它是首个具有一条完整染色体和一个完整质粒的昆虫源该菌菌株的基因组。在所有该菌菌株中，LM1具有最大的基因组。其基因组与来自人类粪便的菌株1001271B_151109_G12的基因组最为接近。我们的结果提供了已知该菌物种和菌株准确的进化关系。基于基因组分析，该菌的泛基因组处于开放状态。大多数该菌菌株具有独特基因，表明这些菌株能够适应不同的生态位和生物体。然而，菌株特异性基因的变异确实与其宿主和生态位存在显著相关性。预计这些菌株产生次生代谢产物的潜力较低。此外，未鉴定出抗生素抗性基因。同时，未发现与毒素产生和分泌系统相关的毒力因子，这表明该菌菌株不足以发挥毒力作用。我们的研究促进了对LM1在蝗虫生物学中的功能及其在蝗虫治理中的潜在应用的发现。
