Ryukyu Animal Medical Center, Yone 5-27, Tomigusuku, Okinawa, 901-0224, Japan.
The United Graduate School of Agriculture Sciences, Kagoshima University, Kagoshima, 890-0065, Japan.
Mycopathologia. 2019 Jun;184(3):393-402. doi: 10.1007/s11046-019-00348-5. Epub 2019 Jun 14.
Recently, we have reported serological cross-reactivity between paracoccidioidomycosis ceti and paracoccidioidomycosis, histoplasmosis, and coccidioidomycosis. However, data on the interaction of Arthrographis kalrae with the above pathogenic fungal infections are lacking. A. kalrae is a widely occurring ascomycetous fungus; causes superficial and deep mycoses; shows thermally dependent dimorphism; and has a genomic profile related to the above-mentioned fungal species. Our study aims to investigate cross-reactivity using eight murine sera, obtained from experimental infection with two A. kalrae isolates. The murine sera were incubated with fungal cells of A. kalrae, Coccidioides posadasii, Histoplasma capsulatum, Paracoccidioides sp., and P. brasiliensis. Thirty murine sera, obtained from experimental infection with six isolates of H. capsulatum, sera from three cases of dolphin paracoccidioidomycosis ceti, two human sera from patients with paracoccidioidomycosis, and a serum sample from a healthy person with a history of coccidioidomycosis, were also incubated with A. kalrae fungal cells and the respective fungal cells that caused the infection as positive controls. Sera derived from the mice infected with A. kalrae reacted strongly when incubated with the Paracoccidioides sp., P. brasiliensis, and C. posadasii, but no positive reaction was observed against the fungal cells of H. capsulatum. The murine sera infected with three out of six isolates of H. capsulatum, and all cetacean and human serum samples reacted positively with the fungal cells of A. kalrae. The present study demonstrated serological cross-reactions among A. kalrae infection, coccidioidomycosis, paracoccidioidomycosis, paracoccidioidomycosis ceti, and histoplasmosis.
最近，我们报道了海球腔菌与球孢子菌病、组织胞浆菌病和粗球孢子菌病之间的血清学交叉反应。然而，关于 Arthrographis kalrae 与上述致病真菌感染相互作用的数据尚缺乏。A. kalrae 是一种广泛存在的子囊菌；引起浅表和深部真菌感染；表现出热依赖性二态性；并且基因组图谱与上述真菌物种相关。我们的研究旨在使用从两株 A. kalrae 分离株实验感染获得的 8 只鼠血清来研究交叉反应。将鼠血清与 A. kalrae、Coccidioides posadasii、Histoplasma capsulatum、Paracoccidioides sp. 和 P. brasiliensis 的真菌细胞孵育。还将来自 6 株 H. capsulatum 分离株实验感染的 30 只鼠血清、3 例海豚海球腔菌病血清、2 例人类副球孢子菌病血清和 1 例来自曾患有粗球孢子菌病的健康人血清与 A. kalrae 真菌细胞和引起感染的相应真菌细胞孵育，作为阳性对照。感染 A. kalrae 的鼠血清与 Paracoccidioides sp.、P. brasiliensis 和 C. posadasii 孵育时反应强烈，但与 H. capsulatum 的真菌细胞无阳性反应。6 株 H. capsulatum 分离株中的 3 株感染的鼠血清以及所有鲸类和人类血清均与 A. kalrae 的真菌细胞呈阳性反应。本研究表明 A. kalrae 感染、球孢子菌病、副球孢子菌病、海球腔菌病和组织胞浆菌病之间存在血清学交叉反应。
