Kostyleva Elena V, Sereda Anna S, Osipov Dmitrii O, Velikoretskaya Irina A, Tsurikova Nina V
All-Russian Research Institute of Food Biotechnology-A Branch of FRC of Food, Biotechnology, and Food Safety, Moscow, Russia.
Federal Research Centre "Fundamentals of Biotechnology" of the Russian Academy of Sciences, Moscow, Russia.
Microbiol Insights. 2019 May 27;12:1178636119848368. doi: 10.1177/1178636119848368. eCollection 2019.
The filamentous fungus is traditionally used as the main industrial producer of cellulases and hemicellulases. Recently, the relevance of carbohydrases hydrolyzing nonstarch polysaccharides of cereals has significantly increased in feed production. In processing of grain raw materials, endodepolymerases, mainly xylanases and endoglucanases, play a key role. Earlier, we carried out gamma mutagenesis of an industrial strain BCM18.2/KK to increase the proportion of endodepolymerases in its enzyme complex. Endoglucanase activity of the strain was increased 5-fold, while xylanase activity increased more than 8-fold. It was interesting to determine the carbohydrases composition in enzyme preparations obtained from the original and mutant strains. So, the strains were cultured in laboratory fermenters; concentrated preparations were obtained using freeze dryer. It was established that gamma mutagenesis resulted in significant changes in the carbohydrases complex of the strain. Cellobiohydrolase I being the major carbohydrase in the original strain was absent in the enzyme complex of the mutant. The share of xylanases and endoglucanases in the preparation from the mutant strain increased by 6% and 6.5%, respectively, compared with the preparation from the original strain. The obtained data show the ability of gamma irradiation to affect the component composition of carbohydrase complex.
丝状真菌传统上是纤维素酶和半纤维素酶的主要工业生产菌。最近，在饲料生产中，水解谷物非淀粉多糖的碳水化合物酶的相关性显著增加。在谷物原料加工过程中，内切解聚酶，主要是木聚糖酶和内切葡聚糖酶，起着关键作用。早些时候，我们对工业菌株BCM18.2/KK进行了γ诱变，以提高其酶复合物中内切解聚酶的比例。该菌株的内切葡聚糖酶活性提高了5倍，而木聚糖酶活性提高了8倍多。确定从原始菌株和突变菌株获得的酶制剂中的碳水化合物酶组成很有意思。因此，将这些菌株在实验室发酵罐中培养；使用冷冻干燥机获得浓缩制剂。结果表明，γ诱变导致该菌株的碳水化合物酶复合物发生了显著变化。原始菌株中的主要碳水化合物酶纤维二糖水解酶I在突变菌株的酶复合物中不存在。与原始菌株的制剂相比，突变菌株制剂中木聚糖酶和内切葡聚糖酶的比例分别增加了6%和6.5%。所得数据表明γ辐射能够影响碳水化合物酶复合物的组成成分。
