Department of Food Science & Biotechnology, Kyungpook National University, Daegu 41566, Korea.
Food and Bio-Industry Research Institute, Inner Beauty/Anti-ageing Center, Kyungpook National University, Daegu 41566, Korea.
Nutrients. 2019 Jun 14;11(6):1341. doi: 10.3390/nu11061341.
It is well known that ultraviolet light activates mitogen-activated protein (MAP) kinase by increasing the reactive oxygen species (ROS) in the body, enhancing activating protein 1(AP-1) complexes (c-Jun and c-Fos), increasing matrix metalloproteinases (MMPs) and degrading collagen and elastin. In this study, we confirmed that polyphenolic rich (SS) stem extracts suppressed ultraviolet (UV)-induced photo-aging. The major active components of SS stem extracts were identified as gallic acid, catechin, vanillic acid, syringic acid and epicatechin. The aqueous and ethanolic extracts of the stem of SS (SSW and SSE, respectively) significantly reduced the elastase enzyme activity. Moreover, both extracts were suppressed the ROS generation and cellular damage induced by UVB in HaCaT cells. Our results also revealed that SSE could regulate the expression of MMPs, tissue inhibitor of matrix metalloproteinase (TIMP)-1, collagen type I alpha 1 (COL1A1), elastin (ELN) and hyaluronan synthase 2 (HAS2) at their transcriptional and translational level. Furthermore, SSE was blocked the UVB-induced phosphorylation of mitogen-activated protein kinases (MAPKs), nuclear factor-kappa B (NF-κB) and c-Jun. Moreover, combination of syringic acid, epicatechin and vanillic acid showed strong synergistic effects on elastase inhibition activity, in which the combination index (CI) was 0.28. Overall, these results strongly suggest that the polyphenolics of SSE exert anti-ageing potential as a natural biomaterial to inhibit UVB-induced photo-aging.
众所周知，紫外线通过增加体内的活性氧（ROS）来激活丝裂原激活蛋白（MAP）激酶，增强激活蛋白 1（AP-1）复合物（c-Jun 和 c-Fos），增加基质金属蛋白酶（MMPs）并降解胶原蛋白和弹性蛋白。在这项研究中，我们证实富含多酚的（SS）茎提取物可抑制紫外线（UV）诱导的光老化。SS 茎提取物的主要活性成分被鉴定为没食子酸、儿茶素、香草酸、丁香酸和表儿茶素。SS 茎的水提物和醇提物（SSW 和 SSE）显著降低弹性蛋白酶酶活性。此外，这两种提取物均可抑制 UVB 在 HaCaT 细胞中诱导的 ROS 生成和细胞损伤。我们的结果还表明，SSE 可以调节 MMPs、基质金属蛋白酶抑制剂 1（TIMP-1）、I 型胶原蛋白 α 1（COL1A1）、弹性蛋白（ELN）和透明质酸合酶 2（HAS2）的表达在转录和翻译水平上。此外，SSE 可阻断 UVB 诱导的丝裂原激活蛋白激酶（MAPKs）、核因子-κB（NF-κB）和 c-Jun 的磷酸化。此外，丁香酸、表儿茶素和香草酸的组合在抑制弹性蛋白酶活性方面表现出很强的协同作用，其中组合指数（CI）为 0.28。总体而言，这些结果强烈表明 SSE 的多酚类物质具有作为天然生物材料抑制 UVB 诱导的光老化的抗衰老潜力。
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