Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci. 2019 Jun 14;20(12):2923. doi: 10.3390/ijms20122923.
Excessive abdominal fat deposition is an issue with general concern in broiler production, especially for Chinese native chicken breeds. A high-fat diet (HFD) can induce body weight gained and excessive fat deposition, and genes and pathways participate in fat metabolism and adipogenesis would be influenced by HFD. In order to reveal the main genes and pathways involved in chicken abdominal fat deposition, we used HFD and normal diet (ND) to feed a Chinese native chicken breed, respectively. Results showed that HFD can increase abdominal fat deposition and induce adipocyte hypertrophy. Additionally, we used RNA-sequencing to identify the differentially expressed genes (DEGs) between HFD and ND chickens in liver and abdominal fat. By analyzed these DEGs, we found that the many DEGs were enriched in fat metabolism related pathways, such as peroxisome proliferator-activated receptor (PPAR) signaling, fat digestion and absorption, extracellular matrix (ECM)-receptor interaction, and steroid hormone biosynthesis. Notably, the expression of , which is a binding protein of mRNA, was found to be induced in liver and abdominal fat by HFD. Ectopic expression of in chicken liver-related cell line Leghorn strain M chicken hepatoma (LMH) cell revealed that can regulate the expression of genes associated with fatty acid metabolism. In chicken preadipocytes (ICP cell line), we found that can promote adipocyte proliferation and differentiation, and the lipid droplet content would be increased by overexpression of . Taken together, this study provides new insights into understanding the genes and pathways involved in abdominal fat deposition of Chinese native broiler, and is an important candidate gene for the study of fat metabolism and adipogenesis in chicken.
过度的腹部脂肪沉积是肉鸡生产中普遍关注的问题，尤其是对于中国本土鸡种。高脂肪饮食（HFD）可诱导体重增加和脂肪过度沉积，参与脂肪代谢和脂肪生成的基因和途径会受到 HFD 的影响。为了揭示参与鸡腹部脂肪沉积的主要基因和途径，我们分别用 HFD 和正常饮食（ND）喂养中国本土鸡种。结果表明，HFD 可增加腹部脂肪沉积并诱导脂肪细胞肥大。此外，我们使用 RNA 测序来鉴定肝脏和腹部脂肪中 HFD 和 ND 鸡之间的差异表达基因（DEGs）。通过分析这些 DEGs，我们发现许多 DEGs 富集在脂肪代谢相关途径中，如过氧化物酶体增殖物激活受体（PPAR）信号、脂肪消化和吸收、细胞外基质（ECM）-受体相互作用和甾体激素生物合成。值得注意的是，HFD 可诱导肝脏和腹部脂肪中 mRNA 结合蛋白的表达。在鸡肝相关细胞系莱航鸡鸡肝癌（LMH）细胞中外源表达 ，发现 可调节与脂肪酸代谢相关的基因表达。在鸡前体脂肪细胞（ICP 细胞系）中，我们发现 可促进脂肪细胞增殖和分化，而过表达 会增加脂滴含量。总之，本研究为深入了解中国本土肉鸡腹部脂肪沉积涉及的基因和途径提供了新的见解， 是研究鸡脂肪代谢和脂肪生成的重要候选基因。
