Wang Lijian, Leng Li, Ding Ran, Gong Pengfei, Liu Chang, Wang Ning, Li Hui, Du Zhi-Qiang, Cheng Bohan
Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150030, PR China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, PR China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China.
Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150030, PR China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, PR China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China.
J Proteomics. 2021 Jun 15;241:104242. doi: 10.1016/j.jprot.2021.104242. Epub 2021 Apr 23.
Genetic selection for meat production performance of broilers concomitantly causes excessive abdominal fat deposition, accompanied by several adverse effects, such as the reduction of feed conversion efficiency and reproduction performance. Our previous studies have identified important genes regulating chicken fat deposition, using the Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) as an animal model. However, the molecular mechanism underlying fat deposition differences between fat and lean broilers remains largely unknown. Here, we integrated the transcriptome (RNA-Seq) and quantitative proteome (isobaric tags for relative and absolute quantitation, iTRAQ) profiling analyses on abdominal fat tissues from NEAUHLF chicken lines. Differentially expressed genes (2167 DEGs, corrected p-value < 0.01) and differentially abundant proteins (199 DAPs, corrected p-value < 0.05) were identified in lean line compared to fat line. Down-regulated DEGs and DAPs mainly enriched in pathways related to fatty acid metabolism, fatty acid biosynthesis, and PPAR signaling, and interestingly, up-regulated DEGs and DAPs enriched both in lysosome pathway. Moreover, numerous key DEGs and DAPs involved in long-chain fatty acid uptake, in situ lipogenesis (fatty acid and cholesterol synthesis), and lipid droplet accumulation were discovered after integrated transcriptome and proteome analysis. SIGNIFICANCE: Excessive abdominal fat deposition critically affects the health of broilers and causes economic loss to broiler producers, but the molecular mechanism of abdominal fat deposition is still unclear in chicken. We identified key DEGs/DAPs and potential pathways through an integration of chicken abdominal fat tissues transcriptome and proteome analyses. Our findings will facilitate a better revealing the mechanism and provide a novel insight into abdominal fat content discrepancy between the fat and lean chicken lines.
对肉鸡产肉性能进行遗传选择会同时导致过多的腹部脂肪沉积，并伴有多种不良影响，如饲料转化效率和繁殖性能降低。我们之前的研究以东北农业大学对腹脂含量进行双向选择的肉鸡品系（NEAUHLF）为动物模型，鉴定出了调控鸡脂肪沉积的重要基因。然而，脂肪型和瘦肉型肉鸡之间脂肪沉积差异的分子机制仍 largely 未知。在此，我们对 NEAUHLF 鸡品系的腹部脂肪组织进行了转录组（RNA 测序）和定量蛋白质组（相对和绝对定量的等压标签，iTRAQ）分析。与脂肪型品系相比，在瘦肉型品系中鉴定出了差异表达基因（2167 个差异表达基因，校正 p 值 < 0.01）和差异丰富蛋白质（199 个差异丰富蛋白质，校正 p 值 < 0.05）。下调的差异表达基因和差异丰富蛋白质主要富集在与脂肪酸代谢、脂肪酸生物合成和 PPAR 信号通路相关的途径中，有趣的是，上调的差异表达基因和差异丰富蛋白质都富集在溶酶体途径中。此外，在整合转录组和蛋白质组分析后，发现了许多参与长链脂肪酸摄取、原位脂肪生成（脂肪酸和胆固醇合成）以及脂滴积累的关键差异表达基因和差异丰富蛋白质。意义：过多的腹部脂肪沉积严重影响肉鸡健康并给肉鸡生产者造成经济损失，但鸡腹部脂肪沉积的分子机制仍不清楚。我们通过整合鸡腹部脂肪组织转录组和蛋白质组分析鉴定出了关键差异表达基因/差异丰富蛋白质和潜在途径。我们的发现将有助于更好地揭示机制，并为脂肪型和瘦肉型鸡品系之间腹部脂肪含量差异提供新的见解。
